LOCUS       sequence-32126-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  TITLE     pCMV-Flag-hMcl-1
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-Flag-hMcl-1
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgat tacgccaagc tcgaaattaa
       61 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcc accatggatt
      121 acaaggatga cgacgataag gcccgggcgg atccactagt ccagtgtggt ggaattgccc
      181 ttatgtttgg cctcaaaaga aacgcggtaa tcggactcaa cctctactgt gggggggccg
      241 gcttgggggc cggcagcggc ggcgccaccc gcccgggagg gcgacttttg gctacggaga
      301 aggaggcctc ggcccggcga gagatagggg gaggggaggc cggcgcggtg attggcggaa
      361 gcgccggcgc aagccccccg tccaccctca cgccagactc ccggagggtc gcgcggccgc
      421 cgcccattgg cgccgaggtc cccgacgtca ccgcgacccc cgcgaggctg cttttcttcg
      481 cgcccacccg ccgcgcggcg ccgcttgagg agatggaagc cccggccgct gacgccatca
      541 tgtcgcccga agaggagctg gacgggtacg agccggagcc tctcgggaag cggccggctg
      601 tcctgccgct gctggagttg gtcggggaat ctggtaataa caccagtacg gacgggtcac
      661 taccctcgac gccgccgcca gcagaggagg aggacgagtt gtaccggcag tcgctggaga
      721 ttatctctcg gtaccttc
//
