LOCUS       sequence-32127-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     pCMV-Flag-hMcl-1
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-Flag-hMcl-1(S159A)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggtttagtg accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta
       61 aagggaacaa aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg
      121 acgacgataa ggcccgggcg gatccactag tccagtgtgg tggaattgcc cttatgtttg
      181 gcctcaaaag aaacgcggta atcggactca acctctactg tgggggggcc ggcttggggg
      241 ccggcagcgg cggcgccacc cgcccgggag ggcgactttt ggctacggag aaggaggcct
      301 cggcccggcg agagataggg ggaggggagg ccggcgcggt gattggcgga agcgccggcg
      361 caagcccccc gtccaccctc acgccagact cccggagggt cgcgcggccg ccgcccattg
      421 gcgccgaggt ccccgacgtc accgcgaccc ccgcgaggct gcttttcttc gcgcccaccc
      481 gccgcgcggc gccgcttgag gagatggaag ccccggccgc tgacgccatc atgtcgcccg
      541 aagaggagct ggacgggtac gagccggagc ctctcgggaa gcggccggct gtcctgccgc
      601 tgctggagtt ggtcggggaa tctggtaata acaccagtac ggacggggca ctaccctcga
      661 cgccgccgcc agcagaggag gaggacgagt tgtaccggca gtcgctggag attatctctc
      721 ggtaccttcg ggagcaggcc accggcgcca aggacacaaa gccaatgggc aggtctgggg
      781 ccacca
//
