LOCUS       sequence-32138-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC
  TITLE     Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion 
            and are essential for embryonic development.
  JOURNAL   J Cell Biol. 2003 Jan 20. 160(2):189-200.
  PUBMED    12527753
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jan 20. 160(2):189-200."
COMMENT     Sequence Label: Mfn2(K109A)-16xmyc
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(55..84)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(118..147)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(154..183)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(190..219)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(238..267)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(274..303)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(310..339)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(358..387)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(394..423)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(430..459)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(478..507)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(514..543)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(550..579)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(598..627)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(634..663)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(670..699)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagctggaga cgtttaactc aatgatgatg atgatgatgg tcgacggcgc tattcagatc
       61 ctcttctgag atgagttttt gttcgggccc aagcttggta ccgagctcgg atccgttcaa
      121 gtcttcttct gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg
      181 ttcaccgttc aaatcttctt cagaaatcaa cttttgttca cctctagagg atccgttcaa
      241 gtcttcttct gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg
      301 ttcaccgttc aaatcttctt cagaaatcaa cttttgttca cctctagagg atccgttcaa
      361 gtcttcttct gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg
      421 ttcaccgttc aaatcttctt cagaaatcaa cttttgttca cctctagagg atccgttcaa
      481 gtcttcttct gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg
      541 ttcaccgttc aaatcttctt cagaaatcaa cttttgttca cctctagagg atccgttcaa
      601 gtcttcttct gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg
      661 ttcaccgttc aaatcttctt cagaaatcaa cttttgttca cctctagagg atcctctgct
      721 gggctgcagg tactggtgtg tgaacatgtt gagttcgctg tccaaccagc cagctttatt
      781 cctgagcagt ttggctctgc tctgaagtga atccagagcc tcgactttct tgttcatggc
      841 agcaatttcc tgctccagat tatctcgggt gatgtcaact tgctggcaca gatgagcaaa
      901 tgtcccagac aattcctgct ggact
//
