LOCUS       sequence-32141-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector to generate recombinant baculovirus by site-specific 
            transposition into a baculovirus shuttle vector For use with 
            Bac-to-Bac Expression System insect cells for secreted protein 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFHMSP-LIC-N
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     90..152
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             165..182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             204..224
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(248..281)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        296..741
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 tttcgtacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
       61 ccatcgggcg cggatctcgg tccgaaacca tgaaattctt agtcaacgtt gcccttgttt
      121 ttatggtcgt atacatttct tacatctatg cggccgctcc ggaacatcac catcaccatc
      181 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgccatg gatctcctga
      241 aagatccata acttcgtata gcatacatta tacgaagtta tgcggccgcg acgtccacat
      301 atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga
      361 ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag
      421 aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct
      481 atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg
      541 tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa
      601 agggcaaagt gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc
      661 gtaactaact tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac
      721 ataaaaaagg agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta
      781 acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac
      841 caaaagccat ataaggaaac atacggcatt tccca
//
