LOCUS       sequence-32142-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a-LIC
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(128..161)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..621
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 ttccctctga atattttgtt taactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt attatgagtt ctcctcctga
      121 aagatccata acttcgtata gcatacatta tacgaagtta tgcggccgcg acgtccacat
      181 atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga
      241 ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag
      301 aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct
      361 atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg
      421 tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa
      481 agggcaaagt gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc
      541 gtaactaact tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac
      601 ataaaaaagg agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta
      661 acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac
      721 caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa
      781 atccctgaac agcaaaaaaa tgaaaaatat aaagttcctg agttcgattc gtccacaatt
      841 aaaaata
//
