LOCUS       sequence-32143-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression under T7 promoter. Uses 
            infusion based cloning method..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p15TV-L
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(121..154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        169..614
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggccatatga gttctcctcc tgaaagatcc
      121 ataacttcgt atagcataca ttatacgaag ttatgcggcc gcgacgtcca catatacctg
      181 ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa
      241 ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga
      301 tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac
      361 aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa
      421 gtaaatcgcg cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa
      481 agtgtatact ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta
      541 acttgccatc ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaaaa
      601 aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta
      661 ctaccgcact gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc
      721 catataagga aacatacggc atttcccata ttacacgcca tgatatgctg caaatccctg
      781 aacagcaaaa aaatgaaaaa tataaagttc ctgagttcga ttcgtccaca attaaaaata
//
