LOCUS       sequence-32144-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, 
            Atkins WM, Sligar SG
  TITLE     Chapter 11 - Reconstitution of membrane proteins in phospholipid 
            bilayer nanodiscs.
  JOURNAL   Methods Enzymol. 2009;464:211-31. doi: 
            10.1016/S0076-6879(09)64011-8.
  PUBMED    19903557
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2009"; volume: "464:211-31. doi"; pages: " 
            10.1016/S0076-6879(09)64011-8"
COMMENT     Sequence Label: pMSP2
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..67
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggtcat catcatcatc atcacattga
       61 gggacgtctg aagctgttgg acaattggga ctctgttacg tctaccttca gtaaacttcg
      121 cgaacaactg ggccccgtga cgcaggaatt ctgggacaac ctggaaaaag aaaccgaggg
      181 actgcgtcag gaaatgtcca aagatttaga agaggtgaag gccaaggttc agccatatct
      241 cgatgacttt cagaaaaaat ggcaggaaga gatggaatta tatcgtcaaa aggtggaacc
      301 gctgcgtgcg gaactgcaag agggggcacg ccaaaaactc catgagctcc aagagaagct
      361 cagcccatta ggcgaagaaa tgcgcgatcg cgcccgtgca catgttgatg cactccggac
      421 tcatttggcg ccgtattcgg atgaacttcg ccagcgtttg gccgcacgtc tcgaggcgct
      481 gaaagaaaac gggggtgccc gcttggctga gtaccacgcg aaagcgacag aacacctgag
      541 caccttgagc gaaaaagcga aaccggcgct ggaagatcta cgccagggct tattgcctgt
      601 tcttgagagc tttaaagtca gttttctgtc agctctggaa gaatatacta aaaagctgaa
      661 tacccagggt accctgaagc tgttggacaa ttgggactct gttacgtcta ccttcagtaa
      721 acttcgcgaa caactgggcc ccgtgacgc
//
