LOCUS       sequence-321442-        5209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast display plasmid intended for expression of N-terminal fusions 
            with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent 
            green-yellow fluorescent protein for detection of expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDN
FEATURES             Location/Qualifiers
     source          1..5209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(201..875)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(876..1156)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(1177..1196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1257..1275)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1316..1819
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1856..1960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1961..2776
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1961..2776
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2034..2053)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2947..3535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3436..3455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3718..4159
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3718..3835
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4130..4153
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             4616..4627
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4628..4654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4721..4750
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actttgttcc cactgtactt ttagctcgta caaaatacaa tatacttttc atttctccgt
       61 aaacaacatg ttttcccatg taatatcctt ttctattttt cgttccgtta ccaactttac
      121 acatactttt gcggtatttc acaccgcata gatcggcaag tgcacaaaca atacttaaat
      181 aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
      241 gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
      301 aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
      361 ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca
      421 cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct
      481 gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg
      541 aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg
      601 taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag
      661 tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca
      721 ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca
      781 tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat
      841 ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta
      901 atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt
      961 cttttttcga ccgaattaat tcttaatcgg caaaaaaaga aaagctccgg atcaagattg
     1021 tacgtaaggt gacaagctat ttttcaataa agaatatctt ccactactgc catctggcgt
     1081 cataactgca aagtacacat atattacgat gctgttctat taaatgcttc ctatattata
     1141 tatatagtaa tgtcgtgatc tatggtgcac tctcagtaca atctgctctg atgccgcata
     1201 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctcgagac gaaagggcct
     1261 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacggatcg
     1321 cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac
     1381 tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta
     1441 gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag
     1501 cgtactttac atatatattt attagacaag aaaagcagat taaatagata tacattcgat
     1561 taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag
     1621 atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg
     1681 ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat
     1741 ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt aaattataat
     1801 tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg
     1861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagccata ttcaacggga
     1981 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2041 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     2101 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2161 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2221 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
     2281 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2341 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2401 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2461 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     2521 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2581 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2641 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     2701 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     2761 cgatgagttt ttctaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2821 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2881 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2941 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3001 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3061 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3121 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3181 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3241 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3301 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3361 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3421 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3481 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat gaaaaaacgc
     3541 cagcaacgcg gcactttgta actgagctgt cattttgctg gcccagctgg tcgaccgcgg
     3601 ctaagcagta ctggtaccgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa
     3661 accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg
     3721 attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc
     3781 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa
     3841 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg
     3901 ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag
     3961 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac
     4021 agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt
     4081 tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct
     4141 atactttaac gtcaaggaga aaaaaccccg gatcgaattc cctacttcat acattttcaa
     4201 ttaagatgag gttcccatct attttcaccg ctgttgtttt tgctgcttct tctgctttgg
     4261 ctgctccagc taatggtcat atggaagcat aataattaag gcgccttatc tcgagggggg
     4321 cggatcctct ggtggaggtg gttcgggcgg aggtggtaac ggatctaatg gttctggcgg
     4381 tagctctgca caggaactga caactatatg cgagcaaatc ccctcaccaa ctttagaatc
     4441 gacgccgtac tctttgtcaa cgactactat tttggccaac gggaaggcaa tgcaaggagt
     4501 ttttgaatat tacaaatcag taacgtttgt cagtaattgc ggttctcacc cctcaacaac
     4561 tagcaaaggc agccccataa acacacagta tgtttttaag gacaatagct cgacgattga
     4621 aggtagatac ccatacgacg ttccagacta cgctctgcag gctagtggtg gaggaggctc
     4681 tggtggaggt ggtagcggag gcggagggtc ggctagccat gaacagaagt tgatctctga
     4741 agaagatttg atgttagaaa aatttgttgg cacctggaag atcgaatcct ctgaaaattt
     4801 tggtgaatac ttgaaggcta tcggtgcccc aaaagaattg gctgatgctg gtgatgctac
     4861 tactccagtc ttgtacattt ctcaaaagga tggtgataag atgaccgtca agattgaaaa
     4921 cggtccacca acttttttgg atacccaagt ttctttcaag ttgggtgaag aattcgacga
     4981 atttccatcc gatagaagaa agggtgttaa gtccgttgtt aacttgtctg gtgagaagtt
     5041 ggtttacgtt caaaagtggg atggtaaaga aaccacttac gtcagagaaa tcaaggacgg
     5101 taaattggtt gttactttga ccatgggtga tgttgttgct gttagatctt atagaagggc
     5161 ctctgaataa tagagatctg ataacaacag tgtagatgta acaaaatcg
//
