LOCUS       sequence-321448-        2756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS part for MoClo assembly, AATG-TTCG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0296
FEATURES             Location/Qualifiers
     source          1..2756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..73
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(77..120)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             785..832
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     terminator      852..909
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(933..950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1104..1692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1184..1203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1875..1969)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1990..2649)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2564..2583
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
       61 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      121 tggaattcgg tctcaaatga gcaaaggtga agaactgttt accggcgttg tgccgattct
      181 ggtggaactg gatggcgatg tgaacggtca caaattcagc gtgcgtggtg aaggtgaagg
      241 cgatgccacg attggcaaac tgacgctgaa atttatctgc accaccggca aactgccggt
      301 gccgtggccg acgctggtga ccaccctgac ctatggcgtt cagtgtttta gtcgctatcc
      361 ggatcacatg aaacgtcacg atttctttaa atctgcaatg ccggaaggct atgtgcagga
      421 acgtacgatt agctttaaag atgatggcaa atataaaacg cgcgccgttg tgaaatttga
      481 aggcgatacc ctggtgaacc gcattgaact gaaaggcacg gattttaaag aagatggcaa
      541 tatcctgggc cataaactgg aatacaactt taatagccat aatgtttata ttacggcgga
      601 taaacagaaa aatggcatca aagcgaattt taccgttcgc cataacgttg aagatggcag
      661 tgtgcagctg gcagatcatt atcagcagaa taccccgatt ggtgatggtc cggtgctgct
      721 gccggataat cattatctga gcacgcagac cgttctgtct aaagatccga acgaaaaagg
      781 cacgcgggac cacatggttc tgcacgaata tgtgaatgcg gcaggtatta cgggttcgcg
      841 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      901 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1081 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1861 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1921 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1981 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     2041 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     2101 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     2161 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     2221 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     2281 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     2341 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2401 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     2461 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     2521 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     2581 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     2641 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     2701 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaact
//
