LOCUS       sequence-32145-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, 
            Owens RJ
  TITLE     A pipeline for the production of antibody fragments for structural 
            studies using transient expression in HEK 293T cells.
  JOURNAL   Protein Expr Purif. 2008 Nov . 62(1):83-9.
  PUBMED    18662785
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2008 Nov . 62(1):83-9."
COMMENT     Sequence Label: pOPINVL
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    210..231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(246..251,252..269,270..276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    284..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     289..311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     308..324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     308..324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             320..493
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(340..357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(340..356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(349..371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
       61 caatcaaagg agatatacca tggggatcct tcccagccct gggatgcctg cgctgctctc
      121 cctcgtgagc cttctctccg tgctgctgat gggttgcgta gctggtaccg tcgacccgac
      181 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      241 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      301 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
      361 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      421 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      481 cctgaatggc gaatggcgcc aagctttcta gaccagagct cgggctgatg ctgcaccaac
      541 tgtatccatc ttcccaccat ccagtgagca gttaacatct ggaggtgcct cagtcgtgtg
      601 cttcttgaac aacttctacc ccaaagacat caatgtcaag tggaagattg atggcagtga
      661 acgacaaaat ggcgtcctg
//
