LOCUS       sequence-321450-        5231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast display plasmid intended for expression of N-terminal fusions 
            with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 
            fluorescent protein and long 2G linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDNg
FEATURES             Location/Qualifiers
     source          1..5231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(194..868)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(869..1149)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(1170..1189)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1250..1268)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1309..1813
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1850..1954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1955..2770
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1955..2770
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2028..2047)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2941..3529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3430..3449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3712..4153
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3712..3829
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4124..4147
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             4631..4642
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4643..4669
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4736..4765
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcccactgta cttttagctc gtacaaaata caatatactt ttcatttctc cgtaaacaac
       61 atgttttccc atgtaatatc cttttctatt tttcgttccg ttaccaactt tacacatact
      121 tttgcggtat ttcacaccgc atagatcggc aagtgcacaa acaatactta aataaatact
      181 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
      241 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
      301 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg
      361 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
      421 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
      481 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
      541 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
      601 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
      661 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
      721 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
      781 ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
      841 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
      901 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
      961 cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa
     1021 ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact
     1081 gcaaagtaca catatattac gatgctgttc tattaaatgc ttcctatatt atatatatag
     1141 taatgtcgtg atctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1201 cagccccgac acccgccaac acccgctgac gcgccctcga gacgaaaggg cctcgtgata
     1261 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgga tcgcttgcct
     1321 gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt
     1381 ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt
     1441 tacggaatga agaaaaaaaa aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact
     1501 ttacatatat atttattaga caagaaaagc agattaaata gatatacatt cgattaacga
     1561 taagtaaaat gtaaaatcac aggattttcg tgtgtggtct tctacacaga caagatgaaa
     1621 caattcggca ttaatacctg agagcaggaa gagcaagata aaaggtagta tttgttggcg
     1681 atccccctag agtcttttac atcttcggaa aacaaaaact attttttctt taatttcttt
     1741 ttttactttc tatttttaat ttatatattt atattaaaaa atttaaatta taattatttt
     1801 tatagcacgt gatgaaaagg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1921 ataaatgctt caataatatt gaaaaaggaa gagtatgagc catattcaac gggaaacgtc
     1981 ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2041 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
     2101 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2161 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2221 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
     2281 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2341 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2401 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2461 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
     2521 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2581 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2641 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2701 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2761 gtttttctaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2821 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3061 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3541 cgcggcactt tgtaactgag ctgtcatttt gctggcccag ctggtcgacc gcggctaagc
     3601 agtactggta ccgttgtgga aatgtaaaga gccccattat cttagcctaa aaaaaccttc
     3661 tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt acggattaga
     3721 agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt cctcgtcttc
     3781 accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga
     3841 ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac ctggccccac
     3901 aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga ttagtttttt
     3961 agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat taacagatat
     4021 ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc ggtttgtatt
     4081 acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac ctctatactt
     4141 taacgtcaag gagaaaaaac cccggatcga attccctact tcatacattt tcaattaaga
     4201 tgaggttccc atctattttc accgctgttg tttttgctgc ttcttctgct ttggctgctc
     4261 cagctaatgg tcatatggaa gcataataat taaggcgcct tatctcgagg ggggcggatc
     4321 cttctcccaa aagttggaca tcaacttgtt ggataacgtt gtcaactctt catatcatgg
     4381 tgaaggtgtt tctggaggtt ctgcacagga actgacaact atatgcgagc aaatcccctc
     4441 accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg ccaacgggaa
     4501 ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta attgcggttc
     4561 tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt ttaaggacaa
     4621 tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc tgcaggctag
     4681 tggtggagga ggctctggtg gaggtggtag cggaggcgga gggtcggcta gccatgaaca
     4741 gaagttgatc tctgaagaag atttgatgtt agaaaaattt gttggcacct ggaagatcga
     4801 atcctctgaa aattttggtg aatacttgaa ggctatcggt gccccaaaag aattggctga
     4861 tgctggtgat gctactactc cagtcttgta catttctcaa aaggatggtg ataagatgac
     4921 cgtcaagatt gaaaacggtc caccaacttt tttggatacc caagtttctt tcaagttggg
     4981 tgaagaattc gacgaatttc catccgatag aagaaagggt gttaagtccg ttgttaactt
     5041 gtctggtgag aagttggttt acgttcaaaa gtgggatggt aaagaaacca cttacgtcag
     5101 agaaatcaag gacggtaaat tggttgttac tttgaccatg ggtgatgttg ttgctgttag
     5161 atcttataga agggcctctg aataatagag atctgataac aacagtgtag atgtaacaaa
     5221 atcgactttg t
//
