LOCUS       sequence-321453-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the 
            lysine residues on the position 109, 135, 151 and 178 on Rheb 
            protein are mutated to arginines).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Yao Y, Hong S, Ikeda T, Mori H, MacDougald OA, Nada S, Okada M, 
            Inoki K
  TITLE     Amino Acids Enhance Polyubiquitination of Rheb and Its Binding to 
            mTORC1 by Blocking Lysosomal ATXN3 Deubiquitinase Activity.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):437-451.e6. doi: 
            10.1016/j.molcel.2020.10.004.
  PUBMED    33157014
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "437-451.e6"
COMMENT     Sequence Label: pLJM1-Flag-Rheb-4Rs
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    624..742
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aagggtggtg gtggtggtgc gtcgacgatg ccgcagtcca agtcccggaa gatcgcgatc
       61 ctgggctacc ggtctgtggg gaaatcctca ttgacgattc aatttgttga aggccaattt
      121 gtggactcct acgatccaac catagaaaac acttttacaa agttgatcac agtaaatgga
      181 caagaatatc atcttcaact tgtagacaca gccgggcaag atgaatattc tatctttcct
      241 cagacatact ccatagatat taatggctat attcttgtgt attctgttac atcaatcaaa
      301 agttttgaag tgattaaagt tatccatggc aaattgttgg atatggtggg gagggtacaa
      361 atacctatta tgttggttgg gaataagaaa gacctgcata tggaaagggt gatcagttat
      421 gaagaaggga gggctttggc agaatcttgg aatgcagctt ttttggaatc ttctgctagg
      481 gaaaatcaga ctgctgtgga tgtttttcga aggataattt tggaggcaga aaaaatggac
      541 ggggcagctt cacaaggcag gtcttcatgc tcggtgatgt gagaattctc gacctcgaga
      601 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      661 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaaagaat tacaaaaaca
      721 aattacaaaa attcaaaatt ttcgggttta ttacagg
//
