LOCUS       sequence-321458-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Flag tagged Rheb protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Yao Y, Hong S, Ikeda T, Mori H, MacDougald OA, Nada S, Okada M, 
            Inoki K
  TITLE     Amino Acids Enhance Polyubiquitination of Rheb and Its Binding to 
            mTORC1 by Blocking Lysosomal ATXN3 Deubiquitinase Activity.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):437-451.e6. doi: 
            10.1016/j.molcel.2020.10.004.
  PUBMED    33157014
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "437-451.e6"
COMMENT     Sequence Label: pLJM1-Flag-Rheb
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    673..790
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtatgga ctacaaggat gacgatgaca agggtggtgg
       61 tggtggtgcg tcgacgatgc cgcagtccaa gtcccggaag atcgcgatcc tgggctaccg
      121 gtctgtgggg aaatcctcat tgacgattca atttgttgaa ggccaatttg tggactccta
      181 cgatccaacc atagaaaaca cttttacaaa gttgatcaca gtaaatggac aagaatatca
      241 tcttcaactt gtagacacag ccgggcaaga tgaatattct atctttcctc agacatactc
      301 catagatatt aatggctata ttcttgtgta ttctgttaca tcaatcaaaa gttttgaagt
      361 gattaaagtt atccatggca aattgttgga tatggtgggg aaagtacaaa tacctattat
      421 gttggttggg aataagaaag acctgcatat ggaaagggtg atcagttatg aagaagggaa
      481 agctttggca gaatcttgga atgcagcttt tttggaatct tctgctaaag aaaatcagac
      541 tgctgtggat gtttttcgaa ggataatttt ggaggcagaa aaaatggacg gggcagcttc
      601 acaaggcaag tcttcatgct cggtgatgtg agaattctcg acctcgagac aaatggcagt
      661 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      721 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      781 tcaaaatttt cgggtttatt acagg
//
