LOCUS       sequence-321459-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all 
            the lysine residues on Rheb except the K19 and K120 are mutated to 
            arginines)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Yao Y, Hong S, Ikeda T, Mori H, MacDougald OA, Nada S, Okada M, 
            Inoki K
  TITLE     Amino Acids Enhance Polyubiquitination of Rheb and Its Binding to 
            mTORC1 by Blocking Lysosomal ATXN3 Deubiquitinase Activity.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):437-451.e6. doi: 
            10.1016/j.molcel.2020.10.004.
  PUBMED    33157014
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "437-451.e6"
COMMENT     Sequence Label: pLJM1-Flag-Rheb-12Rs
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    405..522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gatgaatatt ctatctttcc tcagacatac tccatagata ttaatggcta tattcttgtg
       61 tattctgtta catcaatcag gagttttgaa gtgattaggg ttatccatgg caggttgttg
      121 gatatggtgg ggagggtaca aatacctatt atgttggttg ggaataagag ggacctgcat
      181 atggaaaggg tgatcagtta tgaagaaggg agggctttgg cagaatcttg gaatgcagct
      241 tttttggaat cttctgctag ggaaaatcag actgctgtgg atgtttttcg aaggataatt
      301 ttggaggcag aaaggatgga cggggcagct tcacaaggca ggtcttcatg ctcggtgatg
      361 tgagaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
      421 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      481 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacaggaca
      541 gcaganatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
      601 cctgggtttg cgcag
//
