LOCUS       sequence-32146-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, 
            Owens RJ
  TITLE     A pipeline for the production of antibody fragments for structural 
            studies using transient expression in HEK 293T cells.
  JOURNAL   Protein Expr Purif. 2008 Nov . 62(1):83-9.
  PUBMED    18662785
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2008 Nov . 62(1):83-9."
COMMENT     Sequence Label: pOPINVL
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             20..193
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(40..57)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(40..56)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(49..71)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             566..583
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(673..692)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    738..793
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(792..811)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatggcgcc aagctttcta gaccagagct cgggctgatg ctgcaccaac
      241 tgtatccatc ttcccaccat ccagtgagca gttaacatct ggaggtgcct cagtcgtgtg
      301 cttcttgaac aacttctacc ccaaagacat caatgtcaag tggaagattg atggcagtga
      361 acgacaaaat ggcgtcctga acagttggac tgatcaggac agcaaagaca gcacctacag
      421 catgagcagc accctcacgt tgaccaagga cgagtatgaa cgacataaca gctatacctg
      481 tgaggccact cacaagacat caacttcacc cattgtcaag agcttcaaca ggaatgagtg
      541 ttaaagcttt ctagaccagt ttaaacatca ccatcaccat cactaagtga ttaacctcag
      601 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
      661 cactgagatc gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
      721 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
      781 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
      841 gtatttggtt tagagtttgg caacatatgc ccatatgtaa ctagcataac cc
//
