LOCUS       sequence-32147-e         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, 
            Owens RJ
  TITLE     A pipeline for the production of antibody fragments for structural 
            studies using transient expression in HEK 293T cells.
  JOURNAL   Protein Expr Purif. 2008 Nov . 62(1):83-9.
  PUBMED    18662785
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2008 Nov . 62(1):83-9."
COMMENT     Sequence Label: pOPINVH
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(243..248,249..266,267..273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     286..308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     305..321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     305..321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             317..490
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(337..354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(337..353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(346..368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa
       61 tcaaaggaga tataccatgg ggatccttcc cagccctggg atgcctgcgc tgctctccct
      121 cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct ggtaccgtcg acccgactgg
      181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      301 cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg
      361 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      421 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      481 gaatggcgaa tggcgccaag ctttctagac caaggcgcca aaacgacacc cccatctgtc
      541 tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg
      601 g
//
