LOCUS       sequence-32148-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, 
            Owens RJ
  TITLE     A pipeline for the production of antibody fragments for structural 
            studies using transient expression in HEK 293T cells.
  JOURNAL   Protein Expr Purif. 2008 Nov . 62(1):83-9.
  PUBMED    18662785
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2008 Nov . 62(1):83-9."
COMMENT     Sequence Label: pOPINVH
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..28
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12..28
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             24..197
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(44..61)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(44..60)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..75)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             536..553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(643..662)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    708..763
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(762..781)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
       61 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      121 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      181 gcagcctgaa tggcgaatgg cgccaagctt tctagaccaa ggcgccaaaa cgacaccccc
      241 atctgtctat ccactggccc ctggatctgc tgcccaaact aactccatgg tgaccctggg
      301 atgcctggtc aagggctatt tccctgagcc agtgacagtg acctggaact ctggatccct
      361 gtccagcggt gtgcacacct tcccagctgt cctgcagtct gacctctaca ctctgagcag
      421 ctcagtgact gtcccctcca gcacctggcc cagcgagacc gtcacctgca acgttgccca
      481 cccggccagc agcaccaagg tggacaagaa aattgtgccc agggattgtg gtaaacatca
      541 ccatcaccat cactaagtga ttaacctcag gtgcaggctg cctatcagaa ggtggtggct
      601 ggtgtggcca atgccctggc tcacaaatac cactgagatc gatctttttc cctctgccaa
      661 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      721 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
      781 gagggcaaat catttaaaac atc
//
