LOCUS       sequence-32149-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, 
            Rahman N, Stuart DI, Owens RJ
  TITLE     A versatile ligation-independent cloning method suitable for 
            high-throughput expression screening applications.
  JOURNAL   Nucleic Acids Res. 2007  . 35(6):e45.
  PUBMED    17317681
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(6):e45."
COMMENT     Sequence Label: pOPINF
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    177..198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(213..218,219..236,237..243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    251..267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     256..278
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     275..291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     275..291
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             287..460
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(307..324)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(307..323)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(316..338)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(573..592)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    638..693
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(692..711)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
       61 caatcaaagg agatatacca tggcacacca tcaccaccat cacagcagcg gtctggaagt
      121 tctgtttcag ggtaccgtcg acccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      241 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      301 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      361 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      421 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgccaag ctttctagac
      481 cagtttgtga ttaacctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
      541 atgccctggc tcacaaatac cactgagatc gatctttttc cctctgccaa aaattatggg
      601 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
      661 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
      721 c
//
