LOCUS       sequence-321498-       15684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct conventional Cpf1 vectors that uses U3p and UBI10p to 
            express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant
            genome editing. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15684)
  AUTHORS   Ding D, Chen K, Chen Y, Li H, Xie K
  TITLE     Engineering Introns to Express RNA Guides for Cas9- and 
            Cpf1-Mediated Multiplex Genome Editing.
  JOURNAL   Mol Plant. 2018 Apr 2;11(4):542-552. doi: 
            10.1016/j.molp.2018.02.005. Epub 2018 Feb 17.
  PUBMED    29462720
REFERENCE   2  (bases 1 to 15684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2018.02.005"; journalName: "Mol Plant"; date: 
            "2018-04-2- 2"; volume: "11"; issue: "4"; pages: "542-552"
COMMENT     Sequence Label: p32Fn
FEATURES             Location/Qualifiers
     source          1..15684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..386
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        403..478
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        559..2268
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          1307..2268
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    2279..2303
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2348..2413
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2420..2440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6362..6409
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(6442..6466)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6477..6500
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      6522..6774
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6783..6800)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6783..6799)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6792..6814)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7002..7026
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8327..8956
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9385..10458
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10524..10718
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10954..10976)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11062..11202
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11117..11136
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11217..11234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11388..11976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11468..11487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12063..12857)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13282..13306
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13384..13558
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13599..14624)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14691..15367)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14734..14754)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    15558..15579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15594..15599,15600..15617,15618..15624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15632..15648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15637..15659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15656..15672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15656..15672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttaagga atctttaaac atacgaacag atcacttaaa gttcttctga agcaacttaa
       61 agttatcagg catgcatgga tcttggagga atcagatgtg cagtcaggga ccatagcaca
      121 agacaggcgt cttctactgg tgctaccagc aaatgctgga agccgggaac actgggtacg
      181 ttggaaacca cgtgatgtga agaagtaaga taaactgtag gagaaaagca tttcgtagtg
      241 ggccatgaag cctttcagga catgtattgc agtatgggcc ggcccattac gcaattggac
      301 gacaacaaag actagtatta gtaccacctc ggctatccac atagatcaaa gctgatttaa
      361 aagagttgtg cagatgatcc gtggcaggag accgaggtct cggttttaga gctagaaata
      421 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      481 ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc
      541 atgcctgcag gtccacaaat tcgggtcaag gcggaagcca gcgcgccacc ccacgtcagc
      601 aaatacggag gcgcggggtt gacggcgtca cccggtccta acggcgacca acaaaccagc
      661 cagaagaaat tacagtaaaa aaaaagtaaa ttgcactttg atccaccttt tattacctaa
      721 gtctcaattt ggatcaccct taaacctatc ttttcaattt gggccgggtt gtggtttgga
      781 ctaccatgaa caacttttcg tcatgtctaa cttccctttc agcaaacata tgaaccatat
      841 atagaggaga tcggccgtat actagagctg atgtgtttaa ggtcgttgat tgcacgagaa
      901 aaaaaaatcc aaatcgcaac aatagcaaat ttatctggtt caaagtgaaa agatatgttt
      961 aaaggtagtc caaagtaaaa cttatagata ataaaatgtg gtccaaagcg taattcactc
     1021 aaaaaaaatc aacgagacgt gtaccaaacg gagacaaacg gcatcttctc gaaatttccc
     1081 aaccgctcgc tcgcccgcct cgtcttcccg gaaaccgcgg tggtttcagc gtggcggatt
     1141 ctccaagcag acggagacgt cacggcacgg gactcctccc accacccaac cgccataaat
     1201 accagccccc tcatctcctc tcctcgcatc agctccaccc ccgaaaaatt tctccccaat
     1261 ctcgcgaggc tctcgtcgtc gaatcgaatc ctctcgcgtc ctcaaggtac gctgcttctc
     1321 ctctcctcgc ttcgtttcga ttcgatttcg gacgggtgag gttgttttgt tgctagatcc
     1381 gattggtggt tagggttgtc gatgtgatta tcgtgagatg tttaggggtt gtagatctga
     1441 tggttgtgat ttgggcacgg ttggttcgat aggtggaatc gtggttaggt tttgggattg
     1501 gatgttggtt ctgatgattg gggggaattt ttacggttag atgaattgtt ggatgattcg
     1561 attggggaaa tcggtgtaga tctgttgggg aattgtggaa ctagtcatgc ctgagtgatt
     1621 ggtgcgattt gtagcgtgtt ccatcttgta ggccttgttg cgagcatgtt cagatctact
     1681 gttccgctct tgattgagtt attggtgcca tgggttggtg caaacacagg ctttaatatg
     1741 ttatatctgt tttgtgtttg atgtagatct gtagggtagt tcttcttaga catggttcaa
     1801 ttatgtagct tgtgcgtttc gatttgattt catatgttca cagattagat aatgatgaac
     1861 tcttttaatt aattgtcaat ggtaaatagg aagtcttgtc gctatatctg tcataatgat
     1921 ctcatgttac tatctgccag taatttatgc taagaactat attagaatat catgttacaa
     1981 tctgtagtaa tatcatgtta caatctgtag ttcatctata taatctattg tggtaatttc
     2041 tttttactat ctgtgtgaag attattgcca ctagttcatt ctacttattt ctgaagttca
     2101 ggatacgtgt gctgttacta cctatctgaa tacatgtgtg atgtgcctgt tactatcttt
     2161 ttgaatacat gtatgttctg ttggaatatg tttgctgttt gatccgttgt tgtgtcctta
     2221 atcttgtgct agttcttacc ctatctgttt ggtgattatt tcttgcagat agttatcaac
     2281 aagtttgtac aaaaaagcag gcttcgaagg agatagaacc aattctctaa ggaaatactt
     2341 aaccatggat tataaggatc atgatggaga ttacaaggat cacgatattg attataagga
     2401 tgatgatgat aagatggctc ctaagaagaa gagaaaggtt ggcattcatg gtgtgccggc
     2461 tgcctctatc taccaggagt tcgttaataa gtactctttg tcaaagaccc tcaggtttga
     2521 gcttattcct caaggcaaga ctttggaaaa catcaaggcc agaggtctta ttttggatga
     2581 tgagaagagg gctaaggatt acaagaaggc caagcagatc attgataagt accaccaatt
     2641 ctttatcgag gaaattcttt cttcagtgtg catctcagaa gatctccttc agaattactc
     2701 tgatgtctat ttcaagttga agaagtcaga tgatgataac ctccagaagg attttaagtc
     2761 tgctaaggat actatcaaga agcaaatttc agagtacatc aaggattctg aaaagtttaa
     2821 gaatttgttc aaccagaatc tcattgatgc caagaagggc caagagtcag atctcatcct
     2881 ttggttgaag caatctaagg ataatggtat cgaacttttc aaggctaact cagatatcac
     2941 agatattgat gaggccttgg aaatcattaa gtcttttaag ggctggacta catattttaa
     3001 gggtttccat gagaacagaa agaatgtcta ctcttcaaac gatattccta cttctatcat
     3061 ctacaggatc gttgatgata atttgccaaa gttcctcgag aacaaggcta agtacgaatc
     3121 tctcaaggat aaggctccag aggccattaa ttacgaacag atcaagaagg atcttgccga
     3181 ggaattgaca ttcgatattg attacaagac ctcagaggtt aaccagagag tgttttcttt
     3241 ggatgaggtg ttcgaaatcg ctaacttcaa caattatctc aaccaatcag gcattaccaa
     3301 gttcaatact atcattggtg gcaagtttgt caacggagag aatacaaaga ggaagggcat
     3361 taacgaatac atcaatttgt actctcagca aattaacgat aagacactca agaagtacaa
     3421 gatgtcagtg cttttcaagc agatcttgtc tgataccgag tctaagtcat ttgtcattga
     3481 taagctcgaa gatgattcag atgttgtgac cactatgcag tctttttacg agcaaatcgc
     3541 tgccttcaag acagttgagg aaaagtcaat taaggaaacc ctttctttgc tcttcgatga
     3601 tttgaaggct caaaagctcg atctttcaaa gatctatttc aagaacgata agtcacttac
     3661 cgatttgtct cagcaagtgt ttgatgatta ctctgtcatt ggtactgctg ttcttgagta
     3721 tattacacag caaatcgccc cgaagaacct tgataatcct tcaaagaagg agcaggaatt
     3781 gatcgctaag aagactgaga aggccaagta cttgtctctc gaaactatca agctcgcttt
     3841 ggaggagttc aacaagcata gagatattga taagcaatgc aggtttgagg aaatcctcgc
     3901 caacttcgct gccatcccga tgatttttga tgagatcgct cagaacaagg ataatcttgc
     3961 ccaaatctca attaagtatc agaaccaagg aaagaaggat cttttgcagg cttctgccga
     4021 agatgatgtt aaggctatta aggatctcct tgatcagacc aacaatttgc tccataagct
     4081 caagatcttc cacatttctc aatcagagga taaggctaac atccttgata aggatgaaca
     4141 cttctacttg gttttcgagg aatgttattt cgagcttgcc aacattgtgc cgttgtacaa
     4201 caagatcaga aactacatca ctcagaagcc ttactcagat gagaagttta agctcaactt
     4261 cgaaaattct acccttgcta acggttggga taagaataag gagccagata acactgccat
     4321 cctcttcatt aaggatgata agtactatct tggagtgatg aataagaaga acaataagat
     4381 cttcgatgat aaggctatta aggagaacaa gggcgaaggt tacaagaaga tcgtttacaa
     4441 gctcttgcct ggagctaata agatgttgcc aaaggtgttc ttttcagcca agtctattaa
     4501 gttctacaac ccatctgagg atatcctcag aattaggaat cattcaacac acaccaagaa
     4561 cggatctccg cagaagggct acgagaagtt tgagttcaac atcgaggatt gcagaaagtt
     4621 tattgatttc tataagcaat ctatctcaaa gcatcctgag tggaaggatt ttggcttcag
     4681 attttcagat actcagaggt acaactctat tgatgagttc tatagggagg ttgaaaatca
     4741 aggttacaag ctcacattcg agaacatctc tgaatcatac attgattctg tcgttaacca
     4801 gggaaagctc taccttttcc aaatctacaa caaggatttt tcagcttact ctaagggcag
     4861 accaaatctc cacactcttt attggaaggc cctcttcgat gagaggaatc ttcaggatgt
     4921 ggtctacaag ttgaacggag aggctgaact cttttataga aagcaatcaa ttccaaagaa
     4981 gatcacacat ccggccaagg aggctatcgc caacaagaat aaggataatc ctaagaagga
     5041 gtctgtgttc gaatacgatc ttattaagga taagaggttt acagaggata agttcttttt
     5101 ccactgtcca atcaccatta acttcaagtc ttcaggcgct aacaagttta atgatgagat
     5161 caatctcctt ttgaaggaaa aggccaacga tgttcatatc ctctctattg atagaggaga
     5221 gaggcacctc gcttactata cacttgtgga tggaaagggc aatatcatta agcaggatac
     5281 tttcaacatc attggcaatg ataggatgaa gacaaactac catgataagc tcgctgccat
     5341 tgaaaaggat agagattcag ccaggaagga ttggaagaag atcaacaata ttaaggagat
     5401 gaaggaaggc tacctctctc aagttgtgca cgagattgct aagctcgtta tcgaatataa
     5461 tgccattgtc gttttcgagg atctcaattt cggttttaag agaggaaggt ttaaggtcga
     5521 aaagcaggtt taccaaaagc tcgagaagat gcttatcgaa aagttgaact atctcgtctt
     5581 taaggataac gagttcgata agaccggtgg agttttgagg gcttaccagc tcacagcccc
     5641 gtttgaaacc ttcaagaaga tgggcaagca aactggaatc atctactatg tcccggctgg
     5701 attcacctct aagatctgcc ctgtgactgg ctttgtcaat cagctctacc ctaagtatga
     5761 gtctgtttca aagtctcaag aatttttctc aaagttcgat aagatctgtt acaatcttga
     5821 taagggttac ttcgagtttt ctttcgatta caagaacttc ggagataagg ctgccaaggg
     5881 aaagtggact attgcttcat tcggatctag acttatcaac ttcagaaact ctgataagaa
     5941 ccataactgg gataccagag aggtctatcc aactaaggag ttggaaaagc tccttaagga
     6001 ttactctatc gagtatggtc acggagaatg cattaaggct gccatctgtg gagagtcaga
     6061 taagaagttt ttcgctaagt tgacttctgt gctcaataca atccttcaga tgagaaactc
     6121 aaagactggt acagaacttg attacttgat ttctccggtg gctgatgtca acggaaactt
     6181 cttcgattct aggcaggccc ctaagaatat gccacaagat gctgatgcca acggcgccta
     6241 ccacatcgga ctcaagggcc ttatgttgct cggtaggatt aagaacaatc aagagggaaa
     6301 gaagttgaat ctcgtgatta agaacgagga atattttgag ttcgtccaga acagaaacaa
     6361 taagaggcct gctgccacaa agaaggctgg tcaagctaag aagaagaagt gagaattcgc
     6421 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat aacagcgact
     6481 acaaggatga cgatgacaag gcttagagct cgaatttccc cgatcgttca aacatttggc
     6541 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     6601 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     6661 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     6721 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     6781 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6841 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6901 cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat
     6961 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     7021 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     7081 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     7141 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     7201 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     7261 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     7321 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     7381 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     7441 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     7501 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     7561 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     7621 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     7681 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     7741 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     7801 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     7861 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     7921 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     7981 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     8041 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     8101 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     8161 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     8221 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     8281 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     8341 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     8401 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     8461 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     8521 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     8581 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     8641 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     8701 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     8761 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     8821 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     8881 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     8941 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     9001 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     9061 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     9121 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     9181 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     9241 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgttgagg
     9301 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     9361 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     9421 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     9481 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     9541 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     9601 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     9661 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     9721 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     9781 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     9841 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     9901 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     9961 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
    10021 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
    10081 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
    10141 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
    10201 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
    10261 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
    10321 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
    10381 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
    10441 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
    10501 aaaggtcgaa aagcactctt tcctgtgaat agcacgtaca ttgggaaccc aaagccgtac
    10561 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
    10621 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
    10681 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
    10741 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
    10801 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
    10861 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
    10921 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10981 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11041 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    11101 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    11161 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
    11221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11581 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11821 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
    12061 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
    12121 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
    12181 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
    12241 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
    12301 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
    12361 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
    12421 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
    12481 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
    12541 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
    12601 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
    12661 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
    12721 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
    12781 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
    12841 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
    12901 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
    12961 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
    13021 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
    13081 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
    13141 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
    13201 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    13261 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    13321 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg
    13381 ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa
    13441 gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa
    13501 ccctatagga accctaattc ccttatctgg gaactactca cacattatta tggagaaact
    13561 cgagcttgtc gatcgacaga tcccggtcgg catctactct atttctttgc cctcggacga
    13621 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
    13681 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
    13741 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
    13801 tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc
    13861 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
    13921 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
    13981 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
    14041 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
    14101 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
    14161 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
    14221 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg
    14281 accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
    14341 gaacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc
    14401 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
    14461 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
    14521 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
    14581 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg
    14641 gatctgcgaa agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc
    14701 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
    14761 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
    14821 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    14881 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    14941 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    15001 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    15061 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttcaca
    15121 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    15181 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    15241 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    15301 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    15361 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    15421 agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gcctctcccc
    15481 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    15541 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    15601 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    15661 aacagctatg acatgattac gcca
//
