LOCUS       sequence-321500-       13460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder 
            GFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13460)
  AUTHORS   Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland 
            JG, Ku WL, Veverka V, Zhao K, Crabtree GR
  TITLE     Dominant-negative SMARCA4 mutants alter the accessibility landscape 
            of tissue-unrestricted enhancers.
  JOURNAL   Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 
            10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
  PUBMED    29323272
REFERENCE   2  (bases 1 to 13460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-017-0007-3"; journalName: "Nat Struct Mol Biol"; 
            date: "2018-01"; volume: "25"; issue: "1"; pages: "61-72"
COMMENT     Sequence Label: WT Smarca4-sfGFP
FEATURES             Location/Qualifiers
     source          1..13460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1742..2121
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2123..2398
                     /label=chicken beta-actin promoter
     primer_bind     3339..3361
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(8330..8332,8333..9046)
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8372..8393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     8980..9001
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9077..9665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9130..9150)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9548..9559)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9752..9985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10057..10178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10094..10113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10148..10167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10218..10353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10373..10392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10374..10392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10402..10419)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10402..10418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10411..10433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10560..11015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10647..10666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10857..10878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11041..11145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11146..11214,11215..12006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11364..11383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12177..12765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12666..12685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12919..12936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13053..13074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13089..13094,13095..13112,13113..13119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13127..13143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13132..13154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13151..13167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13151..13167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13186..13206
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13188..13206
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        13234..13460
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatcggcc acgcggatct
     1741 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     1801 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1861 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     1921 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     1981 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2041 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2101 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     2161 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     2221 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
     2281 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     2341 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     2401 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     2461 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     2521 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     2581 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     2641 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     2701 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     2761 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     2821 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     2881 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     2941 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     3001 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     3061 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     3121 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     3181 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     3241 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     3301 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     3361 cggcggctct agccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     3421 ctctctcccc agggggatcc accgggctag catcgccacc atgtctactc cagacccacc
     3481 cttgggtggg actcctcggc ctggtccttc cccaggccct ggtccttcac ctggtgcaat
     3541 gctgggtcct agccctggcc cctcaccagg ttctgcccac agcatgatgg ggccaagccc
     3601 aggacctcct tcagcaggac atcccatgcc cacccagggg cctggagggt acccccagga
     3661 caacatgcat cagatgcaca agcctatgga gtccatgcac gagaagggca tgcctgatga
     3721 cccacgatac aaccagatga aagggatggg catgcggtca ggggcccaca caggcatggc
     3781 acctccacct agtcccatgg accagcattc tcaaggttac ccctcacccc tcggcggctc
     3841 tgaacatgcc tccagtcctg tcccagccag tggcccatct tcaggccccc agatgtcctc
     3901 tgggccagga ggggccccac tagatggttc tgatccccag gccttgggac agcaaaacag
     3961 aggcccaacc ccatttaacc agaaccagct gcatcaactc agagctcaga taatggccta
     4021 caagatgttg gccaggggcc agccattgcc cgaccacctg cagatggccg tgcaaggcaa
     4081 gcggccgatg cctggaatgc agcaacagat gccaacacta cctccaccct cagtgtccgc
     4141 cacaggaccc ggacctggac ccggccctgg ccctggccct ggcccaggac cagcccctcc
     4201 aaattacagt agaccccatg gtatgggagg gcccaacatg cctcccccag gaccctcagg
     4261 tgtgcccccc gggatgcctg gtcagccgcc tggagggcct cccaagccat ggcctgaagg
     4321 acccatggcc aatgctgctg cccccacaag caccccacag aagctgattc ctccgcaacc
     4381 aacaggccgt ccttcacctg cacctcctgc tgtcccgcct gctgcctcac ctgtaatgcc
     4441 accacaaaca cagtccccag ggcagccagc ccagcctgct ccattggtgc cactgcacca
     4501 gaagcagagc cgaatcaccc ccatccagaa gccccgaggc cttgaccctg tggagatcct
     4561 acaagagcgg gagtacaggc ttcaggctcg aatcgcacac agaattcagg aacttgaaaa
     4621 cctccctggg tccctggctg gggaccttcg aaccaaagca accatcgaac tcaaggccct
     4681 taggttgctg aacttccaga ggcagctgcg ccaggaggtg gtggtgtgca tgcgaagaga
     4741 cacagccctg gagacagccc tcaatgccaa ggcctacaag cgcagcaaac gtcagtcact
     4801 acgggaggcc cgcatcactg agaagttgga gaagcagcag aagattgaac aggagcgcaa
     4861 gcgccgccag aagcaccagg agtacctcaa cagcattctg cagcatgcca aggacttcag
     4921 ggagtatcac agatcagtca caggcaaact ccagaaactc accaaggctg tggccaccta
     4981 ccatgccaac actgagcggg agcagaagaa agaaaatgag cgcattgaga aggagcgaat
     5041 gcggaggctt atggctgaag atgaggaggg ctaccgcaaa ctcattgacc agaagaagga
     5101 caagcgcctg gcctaccttc tgcagcagac agatgagtat gtggccaacc tcacagagct
     5161 ggtgcggcag cacaaagctg cccaggttgc caaggagaag aagaagaaaa agaaaaagaa
     5221 gaaggcagaa aatgctgaag gacagacacc tgctattgga ccagatggtg agcctctgga
     5281 tgagaccagc cagatgagtg acctccctgt gaaggtgatc cacgtggaga gtggcaagat
     5341 cctcactggc acagatgccc caaaagccgg gcagctggaa gcctggcttg aaatgaaccc
     5401 agggtatgaa gtagccccca ggtcagacag tgaagaaagt ggctctgaag aggaggagga
     5461 ggaggaggaa gaggagcagc ctcagcccgc acagccccct acactgcctg tggaagaaaa
     5521 gaagaagatt ccagacccag acagcgatga tgtctctgag gtggacgccc gacacattat
     5581 tgagaacgcc aagcaagatg tggacgatga gtacggtgtg tcccaggccc ttgctcgtgg
     5641 cctgcagtct tactatgctg tggcccatgc agtcacagag agagtagata agcagtccgc
     5701 cctcatggtc aacggtgtcc tcaaacagta ccagatcaag ggtttggagt ggctggtgtc
     5761 cctgtacaac aacaacctga atggcatcct ggctgatgag atggggctgg ggaagaccat
     5821 ccagaccatc gcgctcatca catacctcat ggagcacaag cgcatcaacg ggcctttcct
     5881 catcatcgtg cctctctcga cactgtcaaa ctgggcgtat gaatttgaca agtgggcccc
     5941 ctctgtggtg aaggtttctt acaagggctc tccagctgca aggcgagctt ttgtcccaca
     6001 gcttcgcagt gggaagttca acgtcttact gaccacctat gaatatatca tcaaagacaa
     6061 gcatatccta gccaagatcc gctggaagta catgattgtg gatgaaggcc accgcatgaa
     6121 aaaccaccac tgcaagttga cgcaggtcct taacacacac tacgtggccc ctcggcgcct
     6181 gcttcttaca ggcacaccac tgcagaacaa gctaccggag ctctgggccc tgcttaactt
     6241 cctgctcccc actatcttca agagctgcag caccttcgaa cagtggttca atgcaccctt
     6301 tgccatgact ggagaaaagg tggacctgaa tgaagaggag actatcctca ttattcgtcg
     6361 cctacacaaa gttctgcggc ccttcctgct gcggcggctc aagaaggaag ttgaagccca
     6421 gctccctgag aaggtagagt atgtcatcaa atgcgacatg tcagccctgc agcgtgtgct
     6481 gtaccgtcac atgcaggcca aaggtgtgct gctgactgac ggctccgaga aggacaagaa
     6541 gggcaaaggt ggcaccaaga cactgatgaa cactattatg caactgcgta agatctgcaa
     6601 ccacccctac atgttccagc acatcgagga gtccttttct gagcacttgg ggttcaccgg
     6661 cggcatcgtg caaggattgg acctttaccg tgcctcaggg aaatttgaac ttcttgatag
     6721 aattctaccc aaactccgtg caacgaacca taaagtgctc ctcttttgcc aaatgacctc
     6781 cctcatgacc atcatggaag actactttgc ataccgtggc ttcaaatacc tcaggcttga
     6841 tggaaccaca aaagcagaag accggggcat gctgttgaaa acctttaatg aacctggctc
     6901 tgagtatttc attttcctgc tcagtacccg tgctgggggg ctgggcctga atctgcagtc
     6961 agctgacact gtgatcatct ttgacagtga ctggaatccc caccaggacc tgcaagcaca
     7021 ggatcgagcc catcgcattg gacagcagaa tgaggtgcgt gttcttcgcc tgtgcacggt
     7081 caacagtgtg gaagagaaga tactggctgc tgccaaatac aaactcaatg tggatcagaa
     7141 ggtgatccag gcaggcatgt tcgaccagaa gtcgtccagc catgagaggc gtgccttcct
     7201 gcaggccatc ctggagcacg aggagcagga tgaggaggaa gatgaggtgc ctgatgatga
     7261 gaccgtcaac cagatgattg cccggcacga agaagagttt gacctcttca tgcgcatgga
     7321 cttggaccgc cggcgtgaag aagcccgcaa ccccaagcgg aagccacgcc tgatggaaga
     7381 ggatgagctc ccatcctgga tcatcaagga tgatgccgag gtggagcggc tgacatgtga
     7441 agaggaagag gagaagatgt tcggccgtgg ttctcgccac cgcaaggagg tagactacag
     7501 cgactcactg acagagaagc agtggctcaa ggctatcgag gagggcacgc tggaggagat
     7561 cgaagaggag gtccggcaga agaaatcttc acgtaagcgt aagcgagaca gcgaggccgg
     7621 ctcctccacc ccgaccacca gcacccgcag ccgtgacaag gatgaggaga gcaagaagca
     7681 gaagaaacgt gggcggccac ctgctgagaa gctgtcccca aacccaccta acctcaccaa
     7741 gaagatgaag aagatcgtgg atgctgtgat caagtacaaa gacagcagca gtggacgtca
     7801 gctcagcgag gtgttcatcc agctcccctc tcgcaaggag cttcctgagt actatgagct
     7861 catccgaaag cctgtggact tcaagaagat caaggaacgc atccgaaacc acaagtaccg
     7921 cagcctcaat gacctggaga aggatgtgat gctgctgtgc cagaacgctc agacgttcaa
     7981 cctcgagggt tccctgatct atgaggactc catcgtcctg cagtctgtct tcaccagcgt
     8041 acggcagaag attgagaagg aggacgacag tgaaggcgag gaaagcgagg aggaggagga
     8101 gggcgaggag gaaggctccg agtctgagtc ccgctccgtc aaggtgaaga tcaagctggg
     8161 ccgcaaggag aaggcccagg accgactcaa ggggggccgc cggcggccaa gccggggatc
     8221 ccgggccaag ccggttgtga gtgacgatga cagtgaggag gagcaggagg aggaccgctc
     8281 aggaagtggc agtgaggaag acggtggaac cggtagtgga ggatccggtg tgagcaaggg
     8341 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     8401 ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc accaacggca agctgaccct
     8461 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     8521 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     8581 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctccttca aggacgacgg
     8641 cacctacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     8701 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     8761 cttcaacagc cacaacgtct atatcacggc cgacaagcag aagaacggca tcaaggcgaa
     8821 cttcaagatc cgccacaacg tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     8881 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     8941 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     9001 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagtcg acgatatcgt
     9061 acaagtaaga ttcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     9121 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     9181 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     9241 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     9301 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     9361 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     9421 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     9481 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     9541 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     9601 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     9661 cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     9721 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     9781 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     9841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9961 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
    10021 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
    10081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
    10201 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10261 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    10321 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
    10381 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    10441 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    10501 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
    10561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    10621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    10681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    10741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    10801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    10921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10981 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
    11041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    11101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
    11161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
    11221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
    11281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    11341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    11401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    11461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    11521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    11581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    11641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    11701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    11761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    11821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    11881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    11941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    12001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    12061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    12121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    12181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    12241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    12301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    12361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    12421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    12481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    12541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    12601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    12661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    12721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    12781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    12841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    12901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    12961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    13021 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    13081 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    13141 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
    13201 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
    13261 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
    13321 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
    13381 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
    13441 gcctagctcg atacataaac
//
