LOCUS       sequence-321501-       14929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of crRNA array and LbCas12a using one Pol II promoter. 
            For plant genome editing. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Ding D, Chen K, Chen Y, Li H, Xie K
  TITLE     Engineering Introns to Express RNA Guides for Cas9- and 
            Cpf1-Mediated Multiplex Genome Editing.
  JOURNAL   Mol Plant. 2018 Apr 2;11(4):542-552. doi: 
            10.1016/j.molp.2018.02.005. Epub 2018 Feb 17.
  PUBMED    29462720
REFERENCE   2  (bases 1 to 14929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2018.02.005"; journalName: "Mol Plant"; date: 
            "2018-04-2- 2"; volume: "11"; issue: "4"; pages: "542-552"
COMMENT     Sequence Label: p33Lb
FEATURES             Location/Qualifiers
     source          1..14929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    198..219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(234..239,240..257,258..264)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    272..288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     277..299
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     296..312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     296..312
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2079..2103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2160..5843
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5844..5891
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5898..5978
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     protein_bind    complement(6011..6035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6046..6069
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      6091..6343
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6352..6369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6352..6368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6361..6383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6571..6595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7896..8525
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8954..10027
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10093..10287
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10523..10545)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10631..10771
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10686..10705
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10786..10803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10957..11545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11037..11056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11632..12426)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12851..12875
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12953..13127
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13168..14193)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(14260..14929,1..7))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14303..14323)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
       61 agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gcctctcccc
      121 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      181 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      241 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      301 aacagctatg acatgattac gccaagctta caaattcggg tcaaggcgga agccagcgcg
      361 ccaccccacg tcagcaaata cggaggcgcg gggttgacgg cgtcacccgg tcctaacggc
      421 gaccaacaaa ccagccagaa gaaattacag taaaaaaaaa gtaaattgca ctttgatcca
      481 ccttttatta cctaagtctc aatttggatc acccttaaac ctatcttttc aatttgggcc
      541 gggttgtggt ttggactacc atgaacaact tttcgtcatg tctaacttcc ctttcagcaa
      601 acatatgaac catatataga ggagatcggc cgtatactag agctgatgtg tttaaggtcg
      661 ttgattgcac gagaaaaaaa aatccaaatc gcaacaatag caaatttatc tggttcaaag
      721 tgaaaagata tgtttaaagg tagtccaaag taaaacttat agataataaa atgtggtcca
      781 aagcgtaatt cactcaaaaa aaatcaacga gacgtgtacc aaacggagac aaacggcatc
      841 ttctcgaaat ttcccaaccg ctcgctcgcc cgcctcgtct tcccggaaac cgcggtggtt
      901 tcagcgtggc ggattctcca agcagacgga gacgtcacgg cacgggactc ctcccaccac
      961 ccaaccgcca taaataccag ccccctcatc tcctctcctc gcatcagctc cacccccgaa
     1021 aaatttctcc ccaatctcgc gaggctctcg tcgtcgaatc gaatcctctc gcgtcctcaa
     1081 ggtacgctgc ttctcctctc ctcgcttcgt ttcgattcga tttcggacgg gtgaggttgt
     1141 tttgttgcta gatccgattg gtggttaggg ttgtcgatgt gattatcgtg agatgtttag
     1201 gggttgtaga tctgatggtt gtgatttggg cacggttggt tcgataggtg gaatcgtggt
     1261 taggttttgg gattggatgt tggttctgat gattgggggg aatttttacg gttagatgaa
     1321 ttgttggatg attcgattgg ggaaatcggt gtagatctgt tggggaattg tggaactagt
     1381 catgcctgag tgattggtgc gatttgtagc gtgttccatc ttgtaggcct tgttgcgagc
     1441 atgttcagat ctactgttcc gctcttgatt gagttattgg tgccatgggt tggtgcaaac
     1501 acaggcttta atatgttata tctgttttgt gtttgatgta gatctgtagg gtagttcttc
     1561 ttagacatgg ttcaattatg tagcttgtgc gtttcgattt gatttcatat gttcacagat
     1621 tagataatga tgaactcttt taattaattg tcaatggtaa ataggaagtc ttgtcgctat
     1681 atctgtcata atgatctcat gttactatct gccagtaatt tatgctaaga actatattag
     1741 aatatcatgt tacaatctgt agtaatatca tgttacaatc tgtagttcat ctatataatc
     1801 tattgtggta atttcttttt actatctgtg tgaagattat tgccactagt tcattctact
     1861 tatttctgaa gttcaggata cgtgtgctgt tactacctat ctgaatacat gtgtgatgtg
     1921 cctgttacta tctttttgaa tacatgtatg ttctgttgga atatggcagg agaccgaggt
     1981 ctcggtttgc tgtttgatcc gttgttgtgt ccttaatctt gtgctagttc ttaccctatc
     2041 tgtttggtga ttatttcttg cagattctag agttatcaac aagtttgtac aaaaaagcag
     2101 gcttcgaagg agatagaacc aattctctaa ggaaatactt aaccatgggt accgccacca
     2161 tgagcaagct ggagaagttt acaaactgct actccctgtc taagaccctg aggttcaagg
     2221 ccatccctgt gggcaagacc caggagaaca tcgacaataa gcggctgctg gtggaggacg
     2281 agaagagagc cgaggattat aagggcgtga agaagctgct ggatcgctac tatctgtctt
     2341 ttatcaacga cgtgctgcac agcatcaagc tgaagaatct gaacaattac atcagcctgt
     2401 tccggaagaa aaccagaacc gagaaggaga ataaggagct ggagaacctg gagatcaatc
     2461 tgcggaagga gatcgccaag gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga
     2521 aggatatcat cgagacaatc ctgccagagt tcctggacga taaggacgag atcgccctgg
     2581 tgaacagctt caatggcttt accacagcct tcaccggctt ctttgataac agagagaata
     2641 tgttttccga ggaggccaag agcacatcca tcgccttcag gtgtatcaac gagaatctga
     2701 cccgctacat ctctaatatg gacatcttcg agaaggtgga cgccatcttt gataagcacg
     2761 aggtgcagga gatcaaggag aagatcctga acagcgacta tgatgtggag gatttctttg
     2821 agggcgagtt ctttaacttt gtgctgacac aggagggcat cgacgtgtat aacgccatca
     2881 tcggcggctt cgtgaccgag agcggcgaga agatcaaggg cctgaacgag tacatcaacc
     2941 tgtataatca gaaaaccaag cagaagctgc ctaagtttaa gccactgtat aagcaggtgc
     3001 tgagcgatcg ggagtctctg agcttctacg gcgagggcta tacatccgat gaggaggtgc
     3061 tggaggtgtt tagaaacacc ctgaacaaga acagcgagat cttcagctcc atcaagaagc
     3121 tggagaagct gttcaagaat tttgacgagt actctagcgc cggcatcttt gtgaagaacg
     3181 gccccgccat cagcacaatc tccaaggata tcttcggcga gtggaacgtg atccgggaca
     3241 agtggaatgc cgagtatgac gatatccacc tgaagaagaa ggccgtggtg accgagaagt
     3301 acgaggacga tcggagaaag tccttcaaga agatcggctc cttttctctg gagcagctgc
     3361 aggagtacgc cgacgccgat ctgtctgtgg tggagaagct gaaggagatc atcatccaga
     3421 aggtggatga gatctacaag gtgtatggct cctctgagaa gctgttcgac gccgattttg
     3481 tgctggagaa gagcctgaag aagaacgacg ccgtggtggc catcatgaag gacctgctgg
     3541 attctgtgaa gagcttcgag aattacatca aggccttctt tggcgagggc aaggagacaa
     3601 acagggacga gtccttctat ggcgattttg tgctggccta cgacatcctg ctgaaggtgg
     3661 accacatcta cgatgccatc cgcaattatg tgacccagaa gccctactct aaggataagt
     3721 tcaagctgta ttttcagaac cctcagttca tgggcggctg ggacaaggat aaggagacag
     3781 actatcgggc caccatcctg agatacggct ccaagtacta tctggccatc atggataaga
     3841 agtacgccaa gtgcctgcag aagatcgaca aggacgatgt gaacggcaat tacgagaaga
     3901 tcaactataa gctgctgccc ggccctaata agatgctgcc aaaggtgttc ttttctaaga
     3961 agtggatggc ctactataac cccagcgagg acatccagaa gatctacaag aatggcacat
     4021 tcaagaaggg cgatatgttt aacctgaatg actgtcacaa gctgatcgac ttctttaagg
     4081 atagcatctc ccggtatcca aagtggtcca atgcctacga tttcaacttt tctgagacag
     4141 agaagtataa ggacatcgcc ggcttttaca gagaggtgga ggagcagggc tataaggtga
     4201 gcttcgagtc tgccagcaag aaggaggtgg ataagctggt ggaggagggc aagctgtata
     4261 tgttccagat ctataacaag gacttttccg ataagtctca cggcacaccc aatctgcaca
     4321 ccatgtactt caagctgctg tttgacgaga acaatcacgg acagatcagg ctgagcggag
     4381 gagcagagct gttcatgagg cgcgcctccc tgaagaagga ggagctggtg gtgcacccag
     4441 ccaactcccc tatcgccaac aagaatccag ataatcccaa gaaaaccaca accctgtcct
     4501 acgacgtgta taaggataag aggttttctg aggaccagta cgagctgcac atcccaatcg
     4561 ccatcaataa gtgccccaag aacatcttca agatcaatac agaggtgcgc gtgctgctga
     4621 agcacgacga taacccctat gtgatcggca tcgatagggg cgagcgcaat ctgctgtata
     4681 tcgtggtggt ggacggcaag ggcaacatcg tggagcagta ttccctgaac gagatcatca
     4741 acaacttcaa cggcatcagg atcaagacag attaccactc tctgctggac aagaaggaga
     4801 aggagaggtt cgaggcccgc cagaactgga cctccatcga gaatatcaag gagctgaagg
     4861 ccggctatat ctctcaggtg gtgcacaaga tctgcgagct ggtggagaag tacgatgccg
     4921 tgatcgccct ggaggacctg aactctggct ttaagaatag ccgcgtgaag gtggagaagc
     4981 aggtgtatca gaagttcgag aagatgctga tcgataagct gaactacatg gtggacaaga
     5041 agtctaatcc ttgtgcaaca ggcggcgccc tgaagggcta tcagatcacc aataagttcg
     5101 agagctttaa gtccatgtct acccagaacg gcttcatctt ttacatccct gcctggctga
     5161 catccaagat cgatccatct accggctttg tgaacctgct gaaaaccaag tataccagca
     5221 tcgccgattc caagaagttc atcagctcct ttgacaggat catgtacgtg cccgaggagg
     5281 atctgttcga gtttgccctg gactataaga acttctctcg cacagacgcc gattacatca
     5341 agaagtggaa gctgtactcc tacggcaacc ggatcagaat cttccggaat cctaagaaga
     5401 acaacgtgtt cgactgggag gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca
     5461 agtacggcat caattatcag cagggcgata tcagagccct gctgtgcgag cagtccgaca
     5521 aggccttcta ctctagcttt atggccctga tgagcctgat gctgcagatg cggaacagca
     5581 tcacaggccg caccgacgtg gattttctga tcagccctgt gaagaactcc gacggcatct
     5641 tctacgatag ccggaactat gaggcccagg agaatgccat cctgccaaag aacgccgacg
     5701 ccaatggcgc ctataacatc gccagaaagg tgctgtgggc catcggccag ttcaagaagg
     5761 ccgaggacga gaagctggat aaggtgaaga tcgccatctc taacaaggag tggctggagt
     5821 acgcccagac cagcgtgaag cacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
     5881 aaaagaaaaa gggatcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc
     5941 ctgattatgc atacccatat gatgtccccg actatgccta agaattcgcg gccgcactcg
     6001 agatatctag acccagcttt cttgtacaaa gtggttgata acagcgacta caaggatgac
     6061 gatgacaagg cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     6121 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     6181 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     6241 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     6301 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt
     6361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6481 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     6541 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     6601 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     6661 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     6721 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     6781 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     6841 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     6901 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     6961 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     7021 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     7081 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     7141 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     7201 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     7261 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     7321 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     7381 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     7441 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     7501 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     7561 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     7621 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     7681 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     7741 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     7801 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     7861 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     7921 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     7981 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     8041 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     8101 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     8161 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     8221 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     8281 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     8341 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     8401 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     8461 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     8521 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     8581 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     8641 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     8701 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     8761 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     8821 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgttgagga acgggcggtt
     8881 ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc
     8941 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     9001 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     9061 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     9121 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     9181 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     9241 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     9301 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     9361 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     9421 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     9481 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     9541 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     9601 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     9661 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     9721 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     9781 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     9841 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     9901 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     9961 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
    10021 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
    10081 agcactcttt cctgtgaata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
    10141 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
    10201 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
    10261 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
    10321 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
    10381 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
    10441 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
    10501 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10561 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10621 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
    10681 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10741 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
    10801 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10861 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10921 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10981 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11041 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11101 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11161 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11221 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11281 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11341 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    11401 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11461 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11521 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11581 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
    11641 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    11701 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    11761 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    11821 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    11881 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    11941 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    12001 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    12061 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    12121 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    12181 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    12241 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    12301 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    12361 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    12421 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    12481 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    12541 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    12601 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    12661 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    12721 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    12781 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    12841 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    12901 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga
    12961 ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa
    13021 atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa
    13081 ccctaattcc cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg
    13141 atcgacagat cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg
    13201 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    13261 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    13321 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    13381 aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct
    13441 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    13501 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    13561 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    13621 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    13681 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    13741 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    13801 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag
    13861 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga
    13921 gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag
    13981 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    14041 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    14101 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    14161 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa
    14221 gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
    14281 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
    14341 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    14401 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    14461 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    14521 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    14581 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    14641 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt
    14701 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    14761 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    14821 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    14881 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaa
//
