LOCUS       sequence-32152-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, 
            Rahman N, Stuart DI, Owens RJ
  TITLE     A versatile ligation-independent cloning method suitable for 
            high-throughput expression screening applications.
  JOURNAL   Nucleic Acids Res. 2007  . 35(6):e45.
  PUBMED    17317681
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(6):e45."
COMMENT     Sequence Label: pOPINE
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             20..193
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(40..57)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(40..56)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(49..71)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             223..240
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(330..349)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    395..450
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(449..468)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      538..585
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatggcgcc aagctttcta gaccagttta aacatcacca tcaccatcac
      241 taagtgatta acctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
      301 ccctggctca caaataccac tgagatcgat ctttttccct ctgccaaaaa ttatggggac
      361 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
      421 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
      481 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgtaacta
      541 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa agcatgcgga
      601 ggaaattctc cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc
      661 tctgttcact ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc
      721 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataa
//
