LOCUS       sequence-321527-        2598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Vector DK"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Jenni S, Harrison SC
  TITLE     Structure of the DASH/Dam1 complex shows its role at the yeast 
            kinetochore-microtubule interface.
  JOURNAL   Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436.
  PUBMED    29724956
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-4- 4"; volume: "360"; issue: 
            "6388"; pages: "552-558"
COMMENT     Sequence Label: peDK.11
FEATURES             Location/Qualifiers
     source          1..2598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    421..448
                     /gene="telL"
                     /label=telL
                     /note="telomeric sequence from coliphage N15"
     misc_feature    449..476
                     /gene="telR"
                     /label=telR
                     /note="telomeric sequence from coliphage N15"
     protein_bind    complement(634..667)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    699..718
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     727..745
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     protein_bind    856..875
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             1625..2419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQLTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1679..1698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2289..2308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatccatgg gaagcttaaa tgttttacat
       61 ggtcctaatc gggtgcaccg ctcgggcgga ccaaaaccca tgaccactaa aatagagctg
      121 agcctagtcg cgtgatttga cttaagttaa ttaaggccgg ccatttcggt ctgtacgact
      181 atggatcggc aatttgctag acgatctggc acagaaagac acagacgaag gactcactat
      241 ggaatggcgg cttcggccgt caatggcgcg ccgatggatc ccagcggaag taggaagcga
      301 tgtggcctga ggaatacgtc tgtcattgcg tttagataag gcatcccgct ggaaagggaa
      361 ccagtacttt gtaaaggagc tagaggttct ctcgccggcg tcactcggag aatggcaatg
      421 tatcagcaca caatagtcca ttatacgcgc gtataatggg caattgtgtg ctgatacgcc
      481 ggcgttttaa ttaaatgctc tgtggttcgt gcggttctta aaagcaaacg acgagcgaac
      541 tggtgcgtgg gcaaaagggg accctcattt ctaagtacga tcaggtggac tacgattgca
      601 ttttatgggt tccctgcagg aattcctcga gccataactt cgtatagcat acattatacg
      661 aagttatctg ccaggcacat gggtttaaac cgccggcgaa ttgtgagcgc tcacaattgg
      721 cgcgccgcta gttattgctc agcggtggca gcagcctctc gagaggcctt gtactattag
      781 aagtatattt ctagaatcga tacctaggtg ccatttcatt acctctttct ccgcacccga
      841 catagatact cgagtaattg tgagcgctca caattggccg gcccccgggc taagctctca
      901 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
      961 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tggctaacgt
     1021 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
     1081 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
     1141 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
     1201 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac
     1261 atgggaatta gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     1321 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     1381 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     1441 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     1501 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     1561 gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt
     1621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     1681 attcggctat gactgggcac aactgacaat cggctgctct gatgccgccg tgttccggct
     1741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     1801 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     1861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     1921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     1981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     2041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     2101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     2161 cgacggcgag gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga
     2221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     2281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     2341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     2401 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     2461 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     2521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     2581 ttcgcccact acgtaccc
//
