LOCUS       sequence-32153-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, 
            Rahman N, Stuart DI, Owens RJ
  TITLE     A versatile ligation-independent cloning method suitable for 
            high-throughput expression screening applications.
  JOURNAL   Nucleic Acids Res. 2007  . 35(6):e45.
  PUBMED    17317681
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(6):e45."
COMMENT     Sequence Label: pOPINM
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
       61 caatcaaagg agatatacca tggcacacca tcaccaccat cacagcagcg gcatgaaaat
      121 cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga
      181 agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa
      241 actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg
      301 ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga
      361 caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa
      421 gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct
      481 gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa
      541 aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc
      601 tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg
      661 cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaaaca
      721 aacac
//
