LOCUS       sequence-321546-        1483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression GgPCFT protein with C terminally tagged GFP-His tag 
            cleavable with TEV protease..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Parker JL, Deme JC, Kuteyi G, Wu Z, Huo J, Goldman ID, Owens RJ, 
            Biggin PC, Lea SM, Newstead S
  TITLE     Structural basis of antifolate recognition and transport by PCFT.
  JOURNAL   Nature. 2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 
            10.1038/s41586-021-03579-z.
  PUBMED    34040256
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 May 26. pii: 10.1038/s41586-021-03579-z. doi: 
            10.1038/s41586-021-03579-z."
COMMENT     Sequence Label: pDDGFP-Leu2d_GgPCFT
FEATURES             Location/Qualifiers
     source          1..1483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1457..1477
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 accccggatt ctagaactag tggatccccc aaaaatggct gctccatctg atccaccaac
       61 tgctgctact ccaccagctc caccaccacc agctagaaga tgtttgccag ctccatcagt
      121 tgaacctttg ttgtttttgg ctactttggc cttgggttta caagttccat tggctactca
      181 atacttgtgg gatagattgg gtgctgaaag aggttacgtt ggtccaaatg cttcttctcc
      241 acatggttgt ggtaatggtt ctggtgctgt tgatccattg agagaagaag ttgaagcttt
      301 ggttgctcat tggaatttgt gcattaacct aggtggtttc ttcgtcggtt tgttctctgt
      361 tactttgttt ggtccatggt ctgattctgt tggtagaagg ccagttttgg ttttaccagc
      421 tgttggtatg gctgttcaag ctgctgttta tttgttggtc atgtacttga gattgcacgt
      481 tgcttacttg ttgttgggta gaattatctc tggtttgttg ggtgactaca acttgatttt
      541 ggctggttgt tttgcttccg ttgccgattc ttctaatcaa agaactagaa ccttcagagt
      601 cgctattttg gaagcttgtt tgggtgttgc tggtatggtt gcatctgtag gtggtggtca
      661 atggcgtaaa gctgaaggtt atatcaatcc attctggttg gttttggctg cttctttagc
      721 tgctgcatta tatgctgctt tgtgcttgca agaaactgtc aaacaaagaa gggctgctaa
      781 gttgttgaca ttgcaacatt acaaggctgt ctacaagttg tatactgctc cagaagatct
      841 gtcctctaga agaaaattgg ccttgtattc tctggctttc ttcttgttgg ttaccgttca
      901 ttttggtacg aaagacttgt acgtcttgta cgaattgggt tctccattgt gttgggcttc
      961 agatttgatt ggttacggtt ctgctgcttc atacttggct tatttgtcat ctttaggtgg
     1021 cttgaggttg ttgcaattgt gtttggaaga tacttgggtt gccgaaattg gtctgatctc
     1081 taatattgca ggtttggtcg ttatttcttt ggctacaact actccattga tgtttaccgg
     1141 ttacggtatc atgtttttgt ctatggctgc taccccagtt attagagcta aattgtctaa
     1201 gctggtgtcc gaaactgaac aaggtgcttt gttcgcttct gttgcttgtg ttgaaggttt
     1261 gtgttcttta gttgctaccg gtgttttcaa ctccttgtat ccatctacct tgcatttcat
     1321 gagaggtttc ccatttttgt tcggtgccat tttgttgttg attccagctg ctattatggg
     1381 ttggatcgaa atccaagatt ccaacttgca atactcccat ttctctgatg cttcatcttc
     1441 tccagctgat ggtggggaaa atttatattt tcaaggtcaa ttt
//
