LOCUS       sequence-321568-       12294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to construct lentivirus to express membrane-targeted mCherry in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12294)
  TITLE     Expression plasmid for production of murine IFN gamma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mem-mCherry-pTRIP
FEATURES             Location/Qualifiers
     source          1..12294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2932..3639
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3077..3095)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3345..3364
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3707..4206
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3742..3765)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4139..4158
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4227..4826
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4227..4246)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4723..4743
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4840..5428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4893..4913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5311..5322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5714..5894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7422..7441)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7655..7674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7774..7796
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7834..7852)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7920..8024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8025..8093,8094..8885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8243..8262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9056..9644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9545..9564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9798..9815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9885..9905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9890..10219
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10070..10205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11353..11418
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11548..11568
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11993..12127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12030..12049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12084..12103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggggc gataagcttg
     2221 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggccggc
     2821 cgatagttta aacggccggc catgctgtgc tgtatgagaa gaaccaaaca ggttgaaaag
     2881 aatgatgagg accaaaagat cggctgatcg atgctagcgc taccggtcac catggtgagc
     2941 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     3001 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3061 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     3121 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     3181 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3241 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     3301 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     3361 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     3421 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     3481 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     3541 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     3601 ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagaa
     3661 cccgggaagg atccaccgga tctagataac tgatcataat tctaccgggt aggggaggcg
     3721 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     3781 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     3841 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     3901 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     3961 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     4021 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     4081 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     4141 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     4201 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     4261 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     4321 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     4381 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     4441 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     4501 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     4561 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4621 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4681 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4741 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4801 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa
     4861 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4921 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4981 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5041 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5101 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5161 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5221 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     5281 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5341 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5401 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     5461 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     5521 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     5581 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5641 ggctaattca ctcccaacga agacaaaatc gtcgagagat gctgcatata agcagctgct
     5701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5881 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5941 gaaatgaata tcagagagtg agaggccttg acattataat agatttagca ggaattgaac
     6001 taggagtgga gcacacaggc aaagctgcag aagtacttgg aagaagccac cagagatact
     6061 cacgattctg cacatacctg gctaatccca gatcctaagg attacattaa gtttactaac
     6121 atttatataa tgatttatag tttaaagtat aaacttatct aatttactat tctgacagat
     6181 attaattaat cctcaaatat cataagagat gattactatt atccccattt aacacaagag
     6241 gaaactgaga gggaaagatg ttgaagtaat tttcccacaa ttacagcatc cgttagttac
     6301 gactctatga tcttctgaca caaattccat ttactcctca ccctatgact cagtcgaata
     6361 tatcaaagtt atggacatta tgctaagtaa caaattaccc ttttatatag taaatactga
     6421 gtagattgag agaagaaatt gtttgcaaac ctgaatagct tcaagaagaa gagaagtgag
     6481 gataagaata acagttgtca tttaacaagt tttaacaagt aacttggtta gaaagggatt
     6541 caaatgcata aagcaaggga taaatttttc tggcaacaag actatacaat ataaccttaa
     6601 atatgacttc aaataattgt tggaacttga taaaactaat taaatattat tgaagattat
     6661 caatattata aatgtaattt acttttaaaa agggaacata gaaatgtgta tcattagagt
     6721 agaaaacaat ccttattatc acaatttgtc aaaacaagtt tgttattaac acaagtagaa
     6781 tactgcattc aattaagttg actgcagatt ttgtgttttg ttaaaattag aaagagataa
     6841 caacaatttg aattattgaa agtaacatgt aaatagttct acatacgttc ttttgacatc
     6901 ttgttcaatc attgatcgaa gttctttatc ttggaagaat ttgttccaaa gactctgaaa
     6961 taaggaaaac aatctattat atagtctcac acctttgttt tacttttagt gatttcaatt
     7021 taataatgta aatggttaaa atttattctt ctctgagatc atttcacatt gcagatagaa
     7081 aacctgagac tggggtaatt tttattaaaa tctaatttaa tctcagaaac acatctttat
     7141 tctaacatca atttttccag tttgatatta tcatataaag tcagccttcc tcatctgcag
     7201 gttccacaac aaaaatccaa ccaactgtgg atcaaaaata ttgggaaaaa attaaaaata
     7261 gcaatacaac aataaaaaaa tacaaatcag aaaaacagca cagtataaca actttattta
     7321 gcatttacaa tctattaggt attataagta atctagccag atcctctacg ccggacgcat
     7381 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     7441 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     7501 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     7561 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     7621 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7681 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7741 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7801 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     7861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     9901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    10081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    10201 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
    10261 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    10321 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    10381 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    10441 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    10501 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
    10561 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    10621 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    10681 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
    10741 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    10801 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    10861 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
    10921 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    10981 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    11041 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    11101 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    11161 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    11221 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    11281 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    11341 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    11401 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    11461 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    11521 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    11581 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    11641 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    11701 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
    11761 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
    11821 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
    11881 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    11941 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    12001 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    12061 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    12121 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    12181 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    12241 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
