LOCUS       sequence-32157-e         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, 
            Rahman N, Stuart DI, Owens RJ
  TITLE     A versatile ligation-independent cloning method suitable for 
            high-throughput expression screening applications.
  JOURNAL   Nucleic Acids Res. 2007  . 35(6):e45.
  PUBMED    17317681
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(6):e45."
COMMENT     Sequence Label: pOPING
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    213..234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(249..254,255..272,273..279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    287..303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     292..314
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     311..327
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     311..327
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             323..496
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(343..360)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(343..359)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(352..374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             526..543
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa
       61 tcaaaggaga tataccatgg ggatccttcc cagccctggg atgcctgcgc tgctctccct
      121 cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct gaaaccggta ccgtcgaccc
      181 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      241 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      301 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
      361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      421 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      481 cagcctgaat ggcgaatggc gccaagcttt ctagaccagt ttaaacatca ccatcaccat
      541 cactaagtga ttaacctcag gtgcaggctg cctatcagaa ggtggtggct gg
//
