LOCUS       sequence-321571-       12208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to construct lentivirus to express murine FLT3 ligand 
            (variant-1) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12208)
  TITLE     Expression plasmid for production of murine IFN gamma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mu-FLT3Lv1-pTRIP
FEATURES             Location/Qualifiers
     source          1..12208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3621..4120
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3656..3679)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4053..4072
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4141..4740
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4141..4160)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4637..4657
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4754..5342
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4807..4827)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5225..5236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5628..5808
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7336..7355)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7569..7588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7688..7710
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7748..7766)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7834..7938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7939..8007,8008..8799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8157..8176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8970..9558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9459..9478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9712..9729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9799..9819)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9804..10133
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9984..10119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11267..11332
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11462..11482
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11907..12041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11944..11963)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11998..12017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggggc gataagcttg
     2221 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggccggc
     2821 cgatagttta aacgccgcta gcagtcgacc agagatgaca gtgctggcgc cagcctggag
     2881 cccaaattcc tccctgttgc tgctgttgct gctgctgagt ccttgcctgc gggggacacc
     2941 tgactgttac ttcagccaca gtcccatctc ctccaacttc aaagtgaagt ttagagagtt
     3001 gactgaccac ctgcttaaag attacccagt cactgtggcc gtcaatcttc aggacgagaa
     3061 gcactgcaag gccttgtgga gcctcttcct agcccagcgc tggatagagc aactgaagac
     3121 tgtggcaggg tctaagatgc aaacgcttct ggaggacgtc aacaccgaga tacattttgt
     3181 cacctcatgt accttccagc ccctaccaga atgtctgcga ttcgtccaga ccaacatctc
     3241 ccacctcctg aaggacacct gcacacagct gcttgctctg aagccctgta tcgggaaggc
     3301 ctgccagaat ttctctcggt gcctggaggt gcagtgccag ccggactcct ccaccctgct
     3361 gcccccaagg agtcccatag ccctagaagc cacggagctc ccagagcctc ggcccaggca
     3421 gctgttgctc ctgctgctgc tgctgctgcc tctcacactg gtgctgctgg cagccgcctg
     3481 gggccttcgc tggcaaaggg caagaaggag gggggagctc caccctgggg tgcccctccc
     3541 ctcccatccc taggaataaa cgcgtactcg agaacccggg aaggatccac cggatctaga
     3601 taactgatca taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     3661 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     3721 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     3781 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     3841 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     3901 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     3961 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     4021 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     4081 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     4141 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4201 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4261 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4321 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4381 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4441 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4501 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4561 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4621 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4681 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     4741 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4801 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4861 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4921 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4981 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5041 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5101 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5161 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5221 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5281 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5341 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5401 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5461 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5521 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5581 aatcgtcgag agatgctgca tataagcagc tgctttttgc ttgtactggg tctctctggt
     5641 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5701 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5761 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5821 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     5881 cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac aggcaaagct
     5941 gcagaagtac ttggaagaag ccaccagaga tactcacgat tctgcacata cctggctaat
     6001 cccagatcct aaggattaca ttaagtttac taacatttat ataatgattt atagtttaaa
     6061 gtataaactt atctaattta ctattctgac agatattaat taatcctcaa atatcataag
     6121 agatgattac tattatcccc atttaacaca agaggaaact gagagggaaa gatgttgaag
     6181 taattttccc acaattacag catccgttag ttacgactct atgatcttct gacacaaatt
     6241 ccatttactc ctcaccctat gactcagtcg aatatatcaa agttatggac attatgctaa
     6301 gtaacaaatt acccttttat atagtaaata ctgagtagat tgagagaaga aattgtttgc
     6361 aaacctgaat agcttcaaga agaagagaag tgaggataag aataacagtt gtcatttaac
     6421 aagttttaac aagtaacttg gttagaaagg gattcaaatg cataaagcaa gggataaatt
     6481 tttctggcaa caagactata caatataacc ttaaatatga cttcaaataa ttgttggaac
     6541 ttgataaaac taattaaata ttattgaaga ttatcaatat tataaatgta atttactttt
     6601 aaaaagggaa catagaaatg tgtatcatta gagtagaaaa caatccttat tatcacaatt
     6661 tgtcaaaaca agtttgttat taacacaagt agaatactgc attcaattaa gttgactgca
     6721 gattttgtgt tttgttaaaa ttagaaagag ataacaacaa tttgaattat tgaaagtaac
     6781 atgtaaatag ttctacatac gttcttttga catcttgttc aatcattgat cgaagttctt
     6841 tatcttggaa gaatttgttc caaagactct gaaataagga aaacaatcta ttatatagtc
     6901 tcacaccttt gttttacttt tagtgatttc aatttaataa tgtaaatggt taaaatttat
     6961 tcttctctga gatcatttca cattgcagat agaaaacctg agactggggt aatttttatt
     7021 aaaatctaat ttaatctcag aaacacatct ttattctaac atcaattttt ccagtttgat
     7081 attatcatat aaagtcagcc ttcctcatct gcaggttcca caacaaaaat ccaaccaact
     7141 gtggatcaaa aatattggga aaaaattaaa aatagcaata caacaataaa aaaatacaaa
     7201 tcagaaaaac agcacagtat aacaacttta tttagcattt acaatctatt aggtattata
     7261 agtaatctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     7321 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     7381 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     7441 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     7501 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     7561 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7621 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7681 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7741 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7801 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7861 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7921 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7981 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8041 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8101 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8161 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8221 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8281 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8341 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8401 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8461 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8521 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8581 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8641 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8701 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8761 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8821 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8881 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8941 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9001 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9061 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9121 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9181 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9361 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9481 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9541 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9601 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9661 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9721 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9781 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     9841 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     9901 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9961 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    10021 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10081 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
    10141 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
    10201 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
    10261 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
    10321 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
    10381 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
    10441 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    10501 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    10561 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    10621 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    10681 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
    10741 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    10801 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
    10861 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    10921 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    10981 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    11041 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
    11101 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
    11161 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
    11221 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
    11281 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
    11341 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
    11401 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
    11461 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
    11521 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
    11581 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
    11641 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
    11701 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
    11761 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
    11821 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    11881 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    11941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12001 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    12061 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    12121 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    12181 gttaaatatg tactacaaac ttagtagt
//
