LOCUS       sequence-321575-       12296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to construct lentivirus to express katushka in mammalian cells 
            with option for a second insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12296)
  TITLE     Expression plasmid for production of murine IFN gamma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: katushka-FMDV-P2A-pTRIP
FEATURES             Location/Qualifiers
     source          1..12296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2845..2854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2851..3555
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             3580..3636
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        3709..4208
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3744..3767)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4141..4160
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4229..4828
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4229..4248)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4725..4745
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4842..5430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4895..4915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5313..5324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5716..5896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7424..7443)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7657..7676)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7776..7798
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7836..7854)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7922..8026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8027..8095,8096..8887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8245..8264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9058..9646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9547..9566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9800..9817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9887..9907)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9892..10221
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10072..10207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11355..11420
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11550..11570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11995..12129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12032..12051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12086..12105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggggc gataagcttg
     2221 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggccggc
     2821 cgatagttta aacgccgcta gcaagccacc atggtgggtg aggatagcgt gctgatcacc
     2881 gagaacatgc acatgaaact gtacatggag ggcaccgtga acgaccacca cttcaagtgc
     2941 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgaagat caaggtggtc
     3001 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
     3061 aaaaccttta tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag
     3121 ggcttcacat gggagaggat caccacatac gaagacgggg gcgtgctgac cgctacccag
     3181 gacaccagcc tccagaacgg ctgcctcatc tacaacgtca agatcaacgg ggtgaacttc
     3241 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag caccgagatg
     3301 ctgtaccccg ctgacagcgg cctgagaggc catagccaga tggccctgaa gctcgtgggc
     3361 gggggctacc tgcactgctc cctcaagacc acatacagat ccaagaaacc cgctaagaac
     3421 ctcaagatgc ccggcttcta cttcgtggac aggagactgg aaagaatcaa ggaggccgac
     3481 aaagagacct acgtcgagca gcacgagatg gctgtggcca ggtactgcga cctgcctagc
     3541 aaactggggc acagcacgcg tgatctcgag ggcagcggcg ccacaaactt ctctctgcta
     3601 aagcaagcag gtgatgttga agaaaacccc gggccttcgc gacctgcagg aaatcgatta
     3661 tcgtcgacaa ggatccaccg gatctagata actgatcata attctaccgg gtaggggagg
     3721 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3781 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     3841 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     3901 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     3961 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     4021 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     4081 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     4141 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     4201 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     4261 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4321 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4381 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4441 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4501 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4561 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4621 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4681 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4741 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4801 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca
     4861 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4921 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4981 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5041 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5101 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5161 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5221 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5281 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5341 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5401 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     5461 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     5521 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     5581 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5641 agggctaatt cactcccaac gaagacaaaa tcgtcgagag atgctgcata taagcagctg
     5701 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5881 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5941 aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag caggaattga
     6001 actaggagtg gagcacacag gcaaagctgc agaagtactt ggaagaagcc accagagata
     6061 ctcacgattc tgcacatacc tggctaatcc cagatcctaa ggattacatt aagtttacta
     6121 acatttatat aatgatttat agtttaaagt ataaacttat ctaatttact attctgacag
     6181 atattaatta atcctcaaat atcataagag atgattacta ttatccccat ttaacacaag
     6241 aggaaactga gagggaaaga tgttgaagta attttcccac aattacagca tccgttagtt
     6301 acgactctat gatcttctga cacaaattcc atttactcct caccctatga ctcagtcgaa
     6361 tatatcaaag ttatggacat tatgctaagt aacaaattac ccttttatat agtaaatact
     6421 gagtagattg agagaagaaa ttgtttgcaa acctgaatag cttcaagaag aagagaagtg
     6481 aggataagaa taacagttgt catttaacaa gttttaacaa gtaacttggt tagaaaggga
     6541 ttcaaatgca taaagcaagg gataaatttt tctggcaaca agactataca atataacctt
     6601 aaatatgact tcaaataatt gttggaactt gataaaacta attaaatatt attgaagatt
     6661 atcaatatta taaatgtaat ttacttttaa aaagggaaca tagaaatgtg tatcattaga
     6721 gtagaaaaca atccttatta tcacaatttg tcaaaacaag tttgttatta acacaagtag
     6781 aatactgcat tcaattaagt tgactgcaga ttttgtgttt tgttaaaatt agaaagagat
     6841 aacaacaatt tgaattattg aaagtaacat gtaaatagtt ctacatacgt tcttttgaca
     6901 tcttgttcaa tcattgatcg aagttcttta tcttggaaga atttgttcca aagactctga
     6961 aataaggaaa acaatctatt atatagtctc acacctttgt tttactttta gtgatttcaa
     7021 tttaataatg taaatggtta aaatttattc ttctctgaga tcatttcaca ttgcagatag
     7081 aaaacctgag actggggtaa tttttattaa aatctaattt aatctcagaa acacatcttt
     7141 attctaacat caatttttcc agtttgatat tatcatataa agtcagcctt cctcatctgc
     7201 aggttccaca acaaaaatcc aaccaactgt ggatcaaaaa tattgggaaa aaattaaaaa
     7261 tagcaataca acaataaaaa aatacaaatc agaaaaacag cacagtataa caactttatt
     7321 tagcatttac aatctattag gtattataag taatctagcc agatcctcta cgccggacgc
     7381 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     7441 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     7501 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     7561 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     7621 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     7681 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7741 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7801 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7861 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9841 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     9901 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9961 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10021 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10081 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10141 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10201 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
    10261 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    10321 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    10381 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    10441 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    10501 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    10561 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    10621 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    10681 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    10741 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    10801 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    10861 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    10921 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    10981 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    11041 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    11101 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    11161 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    11221 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    11281 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    11341 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    11401 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    11461 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    11521 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    11581 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    11641 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    11701 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    11761 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    11821 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    11881 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    11941 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    12001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    12061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    12121 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    12181 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    12241 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
