LOCUS       sequence-32159-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBXh-1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             158..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(228..264)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
ORIGIN
        1 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaccgcc
       61 tatctcgtgt gagataggcg gagatacgaa ctttaagaag gagatatacc catggtcaaa
      121 tggtcagtat tgagcgatat ctagagaatt cgtcgaccat catcatcatc atcattagag
      181 cttgatatca ttcaggacga gcctcagact ccagcgtaac tggactgaaa acaaactaaa
      241 gcgcccttgt ggcgctttag ttttgttccg cggccaccgg ctggctcgct tcgctcggcc
      301 cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca
      361 cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg
      421 gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc
      481 ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca
      541 gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg
      601 ggcagtcgaa gggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg
      661 ggttccaagg gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag
      721 ccccggaggg gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg
      781 caggggtgcc cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta
      841 aaaatcaaca acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata
      901 gctcattgcg t
//
