LOCUS       sequence-321592-       10173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ACX, rXRCC1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Chen L, Park JE, Paa P, Rajakumar PD, Prekop HT, Chew YT, Manivannan
            SN, Chew WL
  TITLE     Programmable C:G to G:C genome editing with CRISPR-Cas9-directed 
            base excision repair proteins.
  JOURNAL   Nat Commun. 2021 Mar 2;12(1):1384. doi: 10.1038/s41467-021-21559-9.
  PUBMED    33654077
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-2"; volume: "12(1):1384. doi"; pages: " 
            10.1038/s41467-021-21559-9"
COMMENT     Sequence Label: pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        148..527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     682..702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     773..792
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        773..791
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             805..1491
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1540..4938,4939..4941,4942..5538,5539,5540..5542,
                     5543..5544,5545..5547,5548..5640)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1540..1563,1564..1566,1567..5640)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7555..7575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7584..7601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7624..7641)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7630..7854
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7925..7941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7925..7941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7938..7960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7949..7965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7973..7979,7980..7997,7998..8003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8018..8039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8156..8173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8327..8915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8407..8426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9086..9877,9878..9946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9709..9728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9947..10051)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10130..10149)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
       61 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      121 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      721 taagcagagc tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga
      781 ctcactatag ggagagccgc caccatgagc tcagagactg gcccagtggc tgtggacccc
      841 acattgagac ggcggatcga gccccatgag tttgaggtat tcttcgatcc gagagagctc
      901 cgcaaggaga cctgcctgct ttacgaaatt aattgggggg gccggcactc catttggcga
      961 catacatcac agaacactaa caagcacgtc gaagtcaact tcatcgagaa gttcacgaca
     1021 gaaagatatt tctgtccgaa cacaaggtgc agcattacct ggtttctcag ctggagccca
     1081 tgcggcgaat gtagtagggc catcactgaa ttcctgtcaa ggtatcccca cgtcactctg
     1141 tttatttaca tcgcaaggct gtaccaccac gctgaccccc gcaatcgaca aggcctgcgg
     1201 gatttgatct cttcaggtgt gactatccaa attatgactg agcaggagtc aggatactgc
     1261 tggagaaact ttgtgaatta tagcccgagt aatgaagccc actggcctag gtatccccat
     1321 ctgtgggtac gactgtacgt tcttgaactg tactgcatca tactgggcct gcctccttgt
     1381 ctcaacattc tgagaaggaa gcagccacag ctgacattct ttaccatcgc tcttcagtct
     1441 tgtcattacc agcgactgcc cccacacatt ctctgggcca ccgggttgaa aagcggcagc
     1501 gagactcccg ggacctcaga gtccgccaca cccgaaagtg ataaaaagta ttctattggt
     1561 ttagccatcg gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct
     1621 tcaaagaaat ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc
     1681 ggtgccctcc tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct
     1741 cggagaaggt atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat
     1801 gagatggcca aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa
     1861 gaggacaaga aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat
     1921 catgaaaagt acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa
     1981 gcggacctga ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt
     2041 ctcattgagg gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta
     2101 gtacaaacct ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg
     2161 aaggctattc ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa
     2221 ttacccggag agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg
     2281 acaccaaatt ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag
     2341 gacacgtacg atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac
     2401 ttatttttgg ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt
     2461 aatactgaga ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat
     2521 caccaagact tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag
     2581 gaaatattct ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt
     2641 caagaggaat tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag
     2701 ttgcttgtaa aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt
     2761 agcattccac atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat
     2821 ttttatccgt tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata
     2881 ccttactatg tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag
     2941 tccgaagaaa cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct
     3001 caatcgttca tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg
     3061 cctaagcaca gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag
     3121 tatgtcactg agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata
     3181 gtagatctgt tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac
     3241 tttaagaaaa ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat
     3301 gcgtcacttg gtacgtatca tgacctccta aagataatta aagataagga cttcctggat
     3361 aacgaagaga atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat
     3421 cgggaaatga ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg
     3481 aaacagttaa agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac
     3541 gggataagag acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc
     3601 gccaatagga actttatgca gctgatccat gatgactctt taaccttcaa agaggatata
     3661 caaaaggcac aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct
     3721 ggttcgccag ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt
     3781 aaggtcatgg gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa
     3841 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt
     3901 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
     3961 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
     4021 gacataaacc gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag
     4081 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
     4141 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
     4201 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
     4261 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca
     4321 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag
     4381 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
     4441 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
     4501 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt
     4561 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag
     4621 ataggcaagg ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg
     4681 gaaatcactc tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag
     4741 acaggtgaaa tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc
     4801 atgccccaag tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa
     4861 tcgattcttc caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg
     4921 aaaaagtacg gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa
     4981 gttgagaagg gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt
     5041 atggagcgct cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag
     5101 gaagtaaaaa aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat
     5161 ggccgaaaac ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta
     5221 ccgtctaaat acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca
     5281 cctgaagata acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa
     5341 atcatagagc aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac
     5401 aaagtattaa gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat
     5461 attatccatt tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac
     5521 acaacgatag atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt
     5581 caccaatcca tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac
     5641 tctggtggtt ctcctgagat aagcctccgc cacgtcgtaa gttgctccag tcaagactcc
     5701 actcatcgag ctgagaactt gttgaaagcc gacacgtaca gaaagtggag gagcgccaaa
     5761 gcaggggaaa aaacgatatc cgtcgttttg cagctggaga aagaggaaca gatacacagc
     5821 gttgacatcg gaaacgatgg cagtgcattt gtagaagttt tggttggtag tagcgcagga
     5881 ggcgctacgg ccggtgagca agactatgaa gtgctcttgg tgaccagctc tttcatgtcc
     5941 ccgtctgaaa gccgaagcgg gtctaaccca aatagggtcc gaatattcgg gccggataaa
     6001 ttggttagag ccgctgctga gaagaggtgg gaccgcgtta agattgtatg ttcacagccg
     6061 tattccaagg acagcccgta tgggctctca tttgttaaat ttcatagccc cccagataag
     6121 gacgaggccg aagctccgtc acagaaggtg actgtaacta aacttggaca attccgagtc
     6181 aaagaggagg atgattcagc gaatagtctc cggcccggtg cgttgttttt caatcgcatc
     6241 aacaaagctg ccagcgcaag tgcctcagac cctgccgggc ctagttatgc cgcagcaact
     6301 ctgcaagcca gttcagctgc gtcaagtgct ctccccgtcc caaaggttgg cggttcttcc
     6361 tcaaaactgc aagagcctcc aaagggcaag cggaagcttg atctgggatt ggaggattca
     6421 aaacctccct ctaaaccttc agcgggacct gcagctctga agagacctaa actcccggtc
     6481 ccgtctcgga cgccggccgc cacaccagca agtactcctg cgcagaaggc agttcccggg
     6541 aaaccaagag gtgagggcac cgaaccgcgc ggggctcggg ctggtcccca agagttgggg
     6601 aagattctcc aaggagtggt ggtcgtactt agtggcttcc agaatccttt ccgctctgaa
     6661 cttcgcgaca aagcgttgga gcttggtgcc aaatacagac cagactggac ccctgactct
     6721 acccatttga tctgtgcatt tgccaatact ccgaaatata gtcaagttct tgggcttggt
     6781 ggtagaattg ttagaaaaga gtgggtattg gactgctacc gaatgcgaag aaggctgcct
     6841 tcacgaaggt acctcatggc tggccttggg tcctcatctg aggacgaggg agatagccac
     6901 tctgagtccg gcgaggatga agcgcctaaa ttgccacgga agaggcccca accgaaggct
     6961 aagacgcaag ccgctgggcc aagttctcca ccaagacctc caacgccaga agagaccaaa
     7021 gccccatcac ccggcccaca ggataactcc gacactgatg gggagcaaag cgagggtcga
     7081 gataatggag cggaggactc cggtgatact gaagatgaac tcaggcgcgt ggcgaagcaa
     7141 cgggaacaaa ggcagccgcc tgcaccagag gaaaatggag aggatccata cgcgggtagt
     7201 acagacgaga atactgattc agaagctcct tccgaggccg acctccccat acccgagctc
     7261 cccgacttct tccagggcaa gcatttcttt ctctacggcg agttccccgg agacgaacga
     7321 aggaaactca tccgatacgt tacagcattt aatggcgagt tggaagatta catgtctgat
     7381 agggtacagt ttgtgatcac cgcgcaagag tgggatccta attttgagga ggccttgatg
     7441 gaaaatccct ctcttgcttt tgttcggccc agatggatat attcttgcaa tgaaaagcaa
     7501 aagttgctcc ctcatcagtt gtatggggtc gtaccccagg catctggtgg ttctcccaag
     7561 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     7621 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7681 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7741 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7801 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7861 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
     7921 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7981 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
     8041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8101 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8161 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8761 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10081 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
    10141 ctgatgccgc atagttaagc cagtatctgc tcc
//
