LOCUS       sequence-32164-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pNIC-ZB
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..222
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             229..249
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        265..710
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg tgtggataac
       61 aagttcaaca aggagcgtcg aagagctcgc cgtgaaattc gccatctgcc gaacctgaac
      121 cgcgaacagc gtcgcgcatt tattcgcagc ctgcgcgatg atccgagcca gagcgcgaac
      181 ctgctggcgg aagcgaagaa gctgaacgat gcgcagccga agggtaccga gaacctgtac
      241 ttccaatcca tggagaccga cgtccacata tacctgccgt tcactattat ttagtgaaat
      301 gagatattat gatattttct gaattgtgat taaaaaggca actttatgcc catgcaacag
      361 aaactataaa aaatacagag aatgaaaaga aacagataga ttttttagtt ctttaggccc
      421 gtagtctgca aatcctttta tgattttcta tcaaacaaaa gaggaaaata gaccagttgc
      481 aatccaaacg agagtctaat agaatgaggt cgaaaagtaa atcgcgcggg tttgttactg
      541 ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg tatactttgg cgtcacccct
      601 tacatatttt aggtcttttt ttattgtgcg taactaactt gccatcttca aacaggaggg
      661 ctggaagaag cagaccgcta acacagtaca taaaaaagga gacatgaacg atgaacatca
      721 aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg
      781 caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taa
//
