LOCUS       sequence-321648-        6812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human TXLNA mammalian expression vector with 3 C-terminal FLAG 
            tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pCMV3Tag8li-hTXLNA-3xFLAG
FEATURES             Location/Qualifiers
     source          1..6812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2320..2391
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(2444..2463)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2445..2463)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2492..2509)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2498..2705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2712..3167)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2849..2870)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3061..3080
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3194..3298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3299..3367,3368..4156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3517..3536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        4250..4395
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             4438..5475
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(5473..5492)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5517..5565
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6116..6704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6605..6624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gctcgagacc
      661 atgaagaacc aagacaaaaa gaacggggct gccaaacaat ccaatccaaa aagcagccca
      721 ggacaaccgg aagcaggacc cgagggagcc caggagcggc ccagccaggc ggctcctgca
      781 gtagaagcag aaggtcccgg cagcagccag gctcctcgga agccggaggg ggctcaagcc
      841 agaacggctc agtctggggc ccttcgtgat gtctctgagg agctgagccg ccaactggaa
      901 gacatactga gcacatactg tgtggacaat aaccaggggg gccccggcga ggatggggca
      961 cagggtgagc cggctgaacc cgaagatgca gagaagtccc ggacctatgt ggcaaggaat
     1021 ggggagcctg aaccaactcc agtagtcaat ggagagaagg aaccctccaa gggggatcca
     1081 aacacagaag agatccggca gagtgacgag gtcggagacc gagaccatcg aaggccacag
     1141 gagaagaaaa aagccaaggg tttggggaag gagatcacgt tgctgatgca gacattgaat
     1201 actctgagta ccccagagga gaagctggct gctctgtgca agaagtatgc tgaactgctg
     1261 gaggagcacc ggaattcaca gaagcagatg aagctcctac agaaaaagca gagccagctg
     1321 gtgcaagaga aggaccacct gtgcggtgag cacagcaagg ccgtcctggc ccgcagcaag
     1381 cttgagagcc tatgccgtga gctgcagcgg cacaaccgct ccctcaagga agaaggtgtg
     1441 cagcgggccc gggaggagga ggagaagcgc aaggaggtga cctcgcactt ccaggtgaca
     1501 ctgaatgaca ttcagctgca gatggaacag cacaatgagc gcaactccaa gctgcgccaa
     1561 gagaacatgg agctggctga gaggctcaag aagctgattg agcagtatga gctgcgcgag
     1621 gagcatatcg acaaagtctt caaacacaag gacctacaac agcagctggt ggatgccaag
     1681 ctccagcagg cccaggagat gctaaaggag gcagaagagc ggcaccagcg ggagaaggat
     1741 tttctcctga aagaggcagt agagtcccag aggatgtgtg agctgatgaa gcagcaagag
     1801 acccacctga agcaacagct tgccctatac acagagaagt ttgaggagtt ccagaacaca
     1861 ctttccaaaa gcagcgaggt attcaccaca ttcaagcagg agatggaaaa gatgactaag
     1921 aagatcaaga agctggagaa agaaaccacc atgtaccggt cccggtggga gagcagcaac
     1981 aaggccctgc ttgagatggc tgaggagaaa acagtccggg ataaagaact ggagggcctg
     2041 caggtaaaaa tccaacggct ggagaagctg tgccgggcac tgcagacaga gcgcaatgac
     2101 ctgaacaaga gggtacagga cctgagtgct ggtggccagg gctccctcac tgacagtggc
     2161 cctgagagga ggccagaggg gcctggggct caagcaccca gctcccccag ggtcacagaa
     2221 gcgccttgct acccaggagc accgagcaca gaagcatcag gccagactgg gcctcaagag
     2281 cccacctccg ccagggccgc ggccgcaatc gatgtcgagg attacaagga tgacgacgat
     2341 aaggactata aggacgatga tgacaaggac tacaaagatg atgacgataa atagggcccg
     2401 gtaccttaat taattaaggt accaggtaag tgtacccaat tcgccctata gtgagtcgta
     2461 ttacaattca ctcgatcggc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     2521 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2581 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2641 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2701 ggggaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2761 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2821 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2881 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2941 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3001 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3061 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3121 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3541 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3841 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4141 actgattaag cattggcctc aggattagtg cttcatcccc gtggcccgtt gctcgcgttt
     4201 gctggcggtg tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac
     4261 cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg
     4321 tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg
     4381 cagcgacccg cttaacagcg tcaacagcgt gccgcagatc ccggggggca atgagatatg
     4441 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     4501 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     4561 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     4621 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     4681 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     4741 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     4801 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     4861 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     4921 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     4981 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5041 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5101 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5161 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5221 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5281 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5341 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5401 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgga tcgggagatg
     5461 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     5521 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc
     5581 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     5641 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     5701 ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag ggacggggtc
     5761 ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg cgtggggtca
     5821 ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg gcatggaccg
     5881 catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc ccgaccccca
     5941 aaaaccaccg cgcggatttc tggcgccgcc ggacgaacta aacctgacta cgacatctct
     6001 gccccttctt cgctggtacg aggagcgctt tgcctcaggt catgaccaaa atcccttaac
     6061 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6781 cccctgattc tgtggataac cgtattaccg cc
//
