LOCUS       sequence-32165-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pNIC-ZB
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg gagaccgtca
      121 atgccaatag gatatcggca ttttcttttg cgtttttatt tgttaactgt taattgtcct
      181 tgttcaagga tgctgtcttt gacaacagat gttttcttgc ctttgatgtt cagcaggaag
      241 ctaggcgcaa acgttgattg tttgtctgcg tagaatcctc tgtttgtcat atagcttgta
      301 atcacgacat tgtttccttt cgcttgaggt acagcgaagt gtgagtaagt aaaggttaca
      361 tcgttaggat caagatccat ttttaacaca aggccagttt tgttcagcgg cttgtatggg
      421 ccagttaaag aattagaaac ataaccaagc atgtaaatat cgttagacgt aatgccgtca
      481 atcgtcattt ttgatccgcg ggagtcagtg aacaggtacc atttgccgtt cattttaaag
      541 acgttcgcgc gttcaatttc atctgttact gtgttagatg caatcagcgg tttcatcact
      601 tttttcagtg tgtaatcatc gtttagctca atcataccga gagcgccgtt tgctaactca
      661 gccgtgcgtt ttttatcgct ttgcagaagt ttttgacttt cttgacggaa gaatgatgtg
      721 cttttgccat agtatgcttt gttaaataaa gattcttcgc cttggtagcc atcttcagtt
      781 ccagtgtttg cttcaaatac taagtatttg tggcctttat cttctacgta gtgaggatct
      841 ctcagcgtat ggttgtcgcc tgagctgtag ttgccttcat cgatgaactg ctgtaca
//
