LOCUS       sequence-321667-       11327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the dexamethasone -inducible expression of PhiC31 phage 
            recombination directionality factor (RDF) gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: 
            pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos 
            (GB1678)
FEATURES             Location/Qualifiers
     source          1..11327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(1157..1176)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      2409..2661
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2921..2940
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    2973..2992
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3025..3044
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3077..3096
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3129..3148
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3181..3200
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        3273..3319
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      4152..4404
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4642..4666
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5967..6596
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7025..8098
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8164..8358
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8594..8616)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8702..8842
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8757..8776
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8857..8874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9028..9616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9108..9127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9704..10495)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11281..11305
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcccatg
      301 attcagcaag ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata
      361 gttcctgcag cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa
      421 cccgaggtgt tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg
      481 accacactca acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg
      541 ataccaggct tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg
      601 atgtttctca tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg
      661 ctctgctttg ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat
      721 gaccaatgta aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat
      781 gaagagtatc tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg
      841 aagagccaag agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc
      901 atcgtcaaaa gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaag
      961 cttctggact ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt
     1021 ttggataaga ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag
     1081 ataccaaaat attcaaatgg aaatatcaaa aagcttctgt ttcatcaaaa atcaatggtg
     1141 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     1201 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggca
     1261 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     1321 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     1381 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     1441 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     1501 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     1561 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     1621 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     1681 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     1741 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     1801 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgct
     1861 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     1921 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     1981 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     2041 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgta
     2101 tcactggtga aaagaaaaac caccctggag ccaacaaatt ctcaagcgct ttcacaacca
     2161 attgcctcct ctaacgttca tgataacttc atgaataatg aaatcacggc tagtaaaatt
     2221 gatgatggta ataattcaaa accactgtca cctggttgga cggaccaaac tgcgtataac
     2281 gcgtttggaa tcactacagg gatgtttaat accactacaa tggatgatgt atataactat
     2341 ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agtgagcttg gaatggatct
     2401 tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     2461 gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     2521 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     2581 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     2641 tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc
     2701 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2761 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2821 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc
     2881 gcgctgtcag gagttgcgaa gatcgatcca ctagtctttc aattgtgagc gctcacaatt
     2941 ctttctcttc cctttcttct ttctagtctt tcaattgtga gcgctcacaa ttctttctct
     3001 tccctttctt ctttctagtc tttcaattgt gagcgctcac aattctttct cttccctttc
     3061 ttctttctag tctttcaatt gtgagcgctc acaattcttt ctcttccctt tcttctttct
     3121 agtctttcaa ttgtgagcgc tcacaattct ttctcttccc tttcttcttt ctagtctttc
     3181 aattgtgagc gctcacaatt ctttctcttc cctttcttct ttctagctcc accgcggtgg
     3241 cggccggccg tctagtggat cgattcccct tcgcaagacc cttcctctat ataaggaagt
     3301 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
     3361 caaacaacat tacaatttac tattctagtc gaaatggcta agagatctat ttgggctggt
     3421 gatgaagata ataagcctaa gaagagagaa acttatgctg atgatactgt tggtagattt
     3481 cattctggtt attctgaaac taatgaaaga ggtaaggttg ttcctgttgc tcttgataag
     3541 tggagaattt ctactggtga acaatctgtt gctgatgctg ttgctcaact ttttggtggt
     3601 actcctgttg aaaatgaaga atctacttct gaaaatttta ttgatgtttt tactgataga
     3661 cctaaggttc ctgttattat tgaagctgat ggtattcatt gggatatgaa gctttggctt
     3721 aatggtaagc ttaagcatca ttgtgatggt tttgattttg tttctcatgc tgatgaagaa
     3781 atgattggtc aaccttgtgg ttgtcctaag ctttttgatg aaagaaaggc tgctgctaag
     3841 gaatatgatg ctcctaatcc tgctattact gttactttta ctcttgctga tgatcctgaa
     3901 cttggtagat ttaagtttca aactggttct tggactcttt ttaaggttct tcatgaagct
     3961 gaagatgatg ttgaaaaagt tggtaagggt ggtgctgttc ttgctaatct tgaacttgaa
     4021 cttgttgaat atactcctaa gagaggtcct atgagaaata agcttgtttc ttattataag
     4081 cctactatta ctgttcttaa gtcttataat gatgctattg ctgattaggc ttggaatgga
     4141 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4201 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4261 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4321 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4381 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     4441 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     4621 ctcgcgcttg agaccgatat ctgacaggat atattggcgg gtaaacctaa gagaaaagag
     4681 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     4741 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     4801 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat
     4861 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     4921 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     4981 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     5041 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     5101 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     5161 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     5221 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     5281 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     5341 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     5401 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     5461 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     5521 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     5581 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     5641 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     5701 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     5761 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     5821 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     5881 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     5941 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     6001 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     6061 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     6121 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     6181 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     6241 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     6301 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     6361 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     6421 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     6481 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     6541 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     6601 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     6661 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     6721 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     6781 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     6841 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     6901 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     6961 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     7021 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     7081 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     7141 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     7201 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     7261 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     7321 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     7381 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     7441 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     7501 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     7561 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     7621 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     7681 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     7741 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     7801 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     7861 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     7921 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     7981 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     8041 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     8101 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt
     8161 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     8221 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     8281 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     8341 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     8401 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     8461 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     8521 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     8581 atgacggtga aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag
     8641 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     8701 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     8761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     8821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     9481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9661 cgaaaactca cgttaaggga ttttggtcat gcattctagg tgattatttg ccgactacct
     9721 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     9781 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     9841 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     9901 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcaccagccc
     9961 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
    10021 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
    10081 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
    10141 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
    10201 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
    10261 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
    10321 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
    10381 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
    10441 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcataatgt
    10501 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
    10561 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
    10621 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
    10681 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
    10741 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
    10801 cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggaa cagaacttat
    10861 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    10921 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    10981 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
    11041 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
    11101 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
    11161 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
    11221 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    11281 tggcaggata tattgtggtg taaacataac gatatcggtc tcagtca
//
