LOCUS       sequence-321668-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of the nptII, Cas9 and DsRed 
            genes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabe-Orts JM, 
            Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M,
            Quintela JC, Fernandez-Del-Carmen A, Granell A, Orzaez D
  TITLE     The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based 
            Multiplex Genome Engineering in Plants.
  JOURNAL   Front Plant Sci. 2021 Jul 1;12:689937. doi: 
            10.3389/fpls.2021.689937. eCollection 2021.
  PUBMED    34276739
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-07-1"; volume: "12:689937. doi"; pages: " 
            10.3389/fpls.2021.689937. eCollection 2021"
COMMENT     Sequence Label: pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos ...
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             52..119
                     /estimated_length=68
     promoter        245..589
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     533..553
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             663..1340
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(843..863)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1255..1278
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    1916..1940
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3241..3870
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4299..5372
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5438..5632
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5868..5890)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5976..6116
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6031..6050
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6131..6148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6302..6890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6382..6401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6978..7769)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8555..8579
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8625..8877)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8917..9711)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9028..9047)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9638..9657
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9745..9924)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aaccaaggca agtaatagag attggagtct ctaagaaagt agttcctact gnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      121 agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg
      181 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
      241 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
      301 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
      361 attgcgataa aggaaaggct atcgttcaag atgcccctgc cgacagtggt cccaaagatg
      421 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      481 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      541 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggact ccggtatttt
      601 tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact attctagtcg
      661 aaatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg
      721 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      781 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      841 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
      901 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      961 aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct
     1021 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga
     1081 cgatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     1141 agacacacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
     1201 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg
     1261 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc
     1321 gccaccacct gttcctgtag gcttcggcca tgctagagtc cgcaaaaatc accagtctct
     1381 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     1441 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     1501 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     1561 ccagtgacct cgctgtcagg agtctcaatg gtaactttac tctttattta accatacatt
     1621 tttttttatt tttttcactt tgttcttcat ccactattgt tctttgttca tcttgaacaa
     1681 aagctccctc cttctttgtt cttcatccac cattgttctt catcaatcat ttcgctgtca
     1741 ggagtctcaa tggtaacttt actctttatt taaccataca ttttttttta tttttttcac
     1801 tttgttcttc atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg
     1861 ttcttcatcc accattgttc ttcatcaatc atttcgctgt catgagaccg gatcctgaca
     1921 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     1981 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     2041 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     2101 gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     2161 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     2221 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     2281 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     2341 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     2401 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     2461 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     2521 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     2581 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     2641 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     2701 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     2761 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     2821 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     2881 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     2941 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     3001 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca
     3061 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     3121 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     3181 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     3241 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     3301 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     3361 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     3421 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     3481 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     3541 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     3601 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     3661 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     3721 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     3781 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     3841 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     3901 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     3961 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     4021 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     4081 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     4141 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     4201 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     4261 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     4321 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     4381 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     4441 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     4501 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     4561 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     4621 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     4681 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     4741 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     4801 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     4861 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     4921 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     4981 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     5041 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     5101 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     5161 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     5221 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     5281 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     5341 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     5401 ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga
     5461 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     5521 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     5581 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     5641 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     5701 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     5761 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     5821 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5881 cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     5941 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     6001 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6061 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     6121 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6181 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6421 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6481 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6721 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6961 tcatgcattc taggtgatta tttgccgact accttggtga tctcgccttt cacgtagtgg
     7021 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     7081 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     7141 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     7201 aacgtaagca ctacatttcg ctcatcacca gcccagtcgg gcggcgagtt ccatagcgtt
     7261 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     7321 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     7381 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     7441 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     7501 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     7561 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     7621 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     7681 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     7741 gatacttcgg cgatcaccgc ttccctcata atgtttaact ttgttttagg gcgactgccc
     7801 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     7861 tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg
     7921 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     7981 gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt
     8041 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc
     8101 gaggcatttc tgtcctggct ggaacagaac ttattatttc cttcctcttt tctacagtat
     8161 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
     8221 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
     8281 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
     8341 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     8401 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     8461 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     8521 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     8581 taacggatcc ggtctcagga gagcgatcag cttgcatgcc ggtcgatcta gtaacataga
     8641 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
     8701 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
     8761 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatggc
     8821 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc
     8881 ttgactctag ctagagtccg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     8941 atagaaggct atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     9001 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     9061 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     9121 catgatattc ggcaagcagg cgtcgccgtg ggtcacgacg agatcctcgc cgtcgggcat
     9181 ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     9241 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcggtgttt
     9301 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     9361 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     9421 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     9481 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt
     9541 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     9601 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     9661 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcctcgatc
     9721 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     9781 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     9841 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
     9901 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     9961 atcggcgggg gtcataccgt gactccctta attctcatgt atctccgtca ggagactaga
    10021 gccaagctga tctcctttgc cccggagatc accatggacg actttctcta tctctacgat
    10081 ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga taatgagaag
    10141 attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgcg
    10201 gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa ataccttccc
    10261 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa
    10321 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
    10381 a
//
