LOCUS       sequence-321669-       11734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for estradiol-inducible exp of the PhiC31 integrase gene. 
            Includes TU for constitutive exp of an estradiol-inducible 
            transcription activator and TU for estradiol-inducible exp of 
            PhiC31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: 
            pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos 
            (GB1677)
FEATURES             Location/Qualifiers
     source          1..11734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(532..784)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1102..1353)
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             complement(1354..2244)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(1357..2292)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     promoter        complement(2334..2513)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2614..2638
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3424..4215
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4303..4891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4792..4811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5045..5062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5077..5217
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5143..5162)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5303..5325
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5561..5755
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5821..6894)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7323..7952)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9253..9277
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(9519..9771)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9798..9818)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9819..11633)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     promoter        complement(join(11708..11734,1..20))
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 atatagagga agggtcttgc gaaggggagc atattacatg gcattaccac catatacata
       61 tccatataca tatccatatc taatcttacc tcgactgctg tatataaaac cagtggttat
      121 atgtacagta ctgctgtata taaaaccagt ggttatatgt acagtacgtc gactgctgta
      181 tataaaacca gtggttatat gtacagtact gctgtatata aaaccagtgg ttatatgtac
      241 agtacgtcga ggggatgata atgcgattag ttttttagcc tcgacctgca gcccaagctc
      301 tcctgacagc gcgagtcggt cccattattg aagcatttat cagggttatt gtctcatgag
      361 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
      421 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
      481 taggcgtatc acgaggccct ttcgtcttca agaattagct tggcaattcc cgatctagta
      541 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
      601 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      661 tcatgcatta catgttaatt attgcatgct taacgtaatt caacagaaat tatatgataa
      721 tcgtcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
      781 gatcgggatc gaagatccat tccaagctca ctcttttttt gggtttggtg gggtatcttc
      841 atcatcgaat agatagttat atacatcatc cattgtagtg gtattaaaca tccctgtagt
      901 gattccaaac gcgttatacg cagtttggtc cgtccaacca ggtgacagtg gttttgaatt
      961 attaccatca tcaattttac tagccgtgat ttcattattc atgaagttat catgaacgtt
     1021 agaggaggca attggttgtg aaagcgcttg agaatttgta cccccgcttc cgccccctcc
     1081 actgccacct gccggttcac cggcagccac acgacctacc agcggcaacc cttcttcctc
     1141 ttcctgcaac agacgaatcc cgcgtgatgc gccggaaaca atttcaataa cgcctttgcg
     1201 tgccagcgcc ttcagatgtt cttcagccgc gtttggggaa cggaacccca aacgctgcgc
     1261 gatttccgca cgcgtcggcg gcatacctgt ctggctgatg tgatcacgga tgagatcaaa
     1321 cacctcttgt tgcctggccg ttaacgcttt cattactgtg gcagggaaac cctctgcctc
     1381 ccccgtgatg taatactttt gcaaggaatg cgacgaagta gagcccgcag tggccaagtg
     1441 gctttggtcc gtttcctcca cggatgcccc tccacggcta gtgggcgcat gtaggcggtg
     1501 ggcgtccagc atctccagca gcaggtcata gaggggcacc acgttcttgc acttcatgct
     1561 gtacagatgc tccatgcctt tgttactcat gtgcctgatg tgggagagga tgaggaggag
     1621 ctgggccagc cgctggtgct gctgctgcag ggtcaggcct gccttggcca tcaggtggat
     1681 caaagtgtct gtgatcttgt ccaggactcg gtggatatgg tccttctctt ccagagactt
     1741 cagggtgctg gacagaaatg tgtacactcc agaattaagc aaaataatag atttgaggca
     1801 cacaaactcc tctccctgca gattcatcat gcggaaccga gatgatgtag ccagcagcat
     1861 gtcgaagatc tccaccatgc cctctacaca ttttccctgg ttcctgtcca agagcaagtt
     1921 aggagcaaac agtagcttcc ctgggtgctc catggagcgc cagacgagtc caatcatcag
     1981 gatctctagc caggcacatt ctagaaggtg gacctgatca tggagggtca aatccacaaa
     2041 gcctggcacc ctcttcgccc agttgatcat gtgaaccagc tccctgtctg ccaggttggt
     2101 cagtaagccc atcatcgaag cttcactgaa gggtctggta ggatcatact cggaatagag
     2161 tatggggggc tcagcatcca acaaggcact gaccatctgg tcggccgtca gggacaaggc
     2221 caggctgttc ttcttagagc gtttgatcat gagcgggctt ggccaaaggt tggcagctct
     2281 catgtctcca gcagacatgg gcctcgatcg agttgagagt gaatatgaga ctctaattgg
     2341 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     2401 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     2461 ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg ttctgtcagt
     2521 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataccgtg actcccttaa
     2581 ttctcatgta tctcctgaga ccggatccgt tatgtttaca ccacaatata tcctgccacc
     2641 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     2701 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     2761 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     2821 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaccgc
     2881 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     2941 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3001 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat
     3061 aagttctgtt ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg
     3121 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     3181 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     3241 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     3301 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     3361 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     3421 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     3481 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     3541 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     3601 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     3661 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     3721 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     3781 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     3841 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     3901 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     3961 gctggtgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     4021 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     4081 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     4141 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     4201 gtagtcggca aataatcacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt
     4261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4381 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4441 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4621 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4741 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4801 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4861 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4921 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4981 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5041 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     5101 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     5161 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     5221 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5281 tacagacaag ctgtgaccgt caccgggagc tgcatgtgtc agaggttttc accgtcatca
     5341 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     5401 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     5461 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     5521 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     5581 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     5641 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     5701 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     5761 gagtcctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     5821 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     5881 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     5941 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     6001 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     6061 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     6121 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     6181 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     6241 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     6301 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     6361 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     6421 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     6481 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     6541 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     6601 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     6661 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     6721 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     6781 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     6841 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     6901 tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca gccgcttacg
     6961 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     7021 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     7081 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     7141 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     7201 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     7261 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     7321 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     7381 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
     7441 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
     7501 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
     7561 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
     7621 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
     7681 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
     7741 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
     7801 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
     7861 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
     7921 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
     7981 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
     8041 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
     8101 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag aggtgcgcgg
     8161 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
     8221 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
     8281 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
     8341 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
     8401 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
     8461 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
     8521 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
     8581 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
     8641 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
     8701 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
     8761 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
     8821 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
     8881 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
     8941 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
     9001 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
     9061 gtcgttttca gatgacggct gcactgaacg tcagaagccg actgcactat agcagcggag
     9121 gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
     9181 tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct
     9241 cttttctctt aggtttaccc gccaatatat cctgtcagga tccggtctca tgacagcgcg
     9301 agtcggtccc attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9361 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9421 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9481 aggccctttc gtcttcaaga attagcttgg caattcccga tctagtaaca tagatgacac
     9541 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
     9601 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
     9661 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatcg tcgcaagacc
     9721 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cgggatcgaa
     9781 gatccattcc aagcttacac cttcctcttt ttttttggtg ctgccacatc ctcggtacca
     9841 tcttgtgcat cgtcttcatc atcatcggtt ggaggctttg cccaggtaat actggctctc
     9901 ttttcgatgg gagtgccctg tcctcgaccc gtagtggact tagtaactac aatcttgtca
     9961 acaaatagac ccacaaacac tctcttatcg tctacgctag ctcttcccca ccagctcttt
    10021 ggacctgtag gatcagcgtc ggcatcctca ggaaaccatt gatcgagtgg aagctttggg
    10081 gcctcggcgg cctcaagctc tgcgagtcgc tcctctgcgc cttgctgtct caaagttagt
    10141 gcagcttgtt gcttcctgaa atgcttccga ccaactggtc catcatatgc tccggcagcc
    10201 ctatcttcgt aaagttcttc caatgcattc aaggcgtctg ctctttcggc taccaaatta
    10261 gctctttcgc cacttttttc tggtgcctct gttagcttac caaaacgccg ggcagcctcc
    10321 caaagaagag ctaaagtttc ctcatcccct tcagcgtgtc taatcttgtt gaaaatacgt
    10381 tcggccacaa atttatcaag agcagccata gaaacgttgc atgtaccctc atgctgtcct
    10441 ggagcagaag ggtccaccac tttacgacgt ctgcacctgt aagaatcctt gattgactct
    10501 tctccacgtt ttgatgtcat aacagcccca cactcacaat aaagcttatc cattgctgac
    10561 aaaattgcct gtccccgaga taaacccttt ccacgccctc taccatcaag ccaggcctgc
    10621 aactcgtacc actcggcagg ttcgatgatc ggtccacagt ctaattcaac tggacgcaaa
    10681 gtaataggat ccctttggat cctataacct tcgatttttg tagtaggagt accatcaggt
    10741 tttttcttat aaatgacttc tgcggcgaat ccagcaatac gcggatcccg aaggattctc
    10801 ataactgtag cgggatccca agcggaagat gcagtcttct taccgattgt ttcccctcga
    10861 gttggcactg cgtcagcatc catcctttta caaagcccag tgatagatcc agggtggata
    10921 gcagcctggc tacctggctt aaatggaaga tgtttgtgag ttttgatctc tctccaccac
    10981 catctaataa cgtcaggttc gaactcaaaa ggccctgtga gaggggttgt gctatgagct
    11041 agcttattaa taaccacgtt caccatccta ccatttctgg taatctcttt tgtctcactc
    11101 acgagttcga aaccatacgg agcttttcct cctacatagc ctccaagctc cctttgcagg
    11161 ttcttggtgt ccaagatttt agcactcttc agtgatgact ctttatggct agcgtcgagt
    11221 cgcataataa ggtgtataag gtccatcaca ttaccctgcc tgaatacacc ttcttgtgta
    11281 cttacgatag taacaccgag tgcaagtagc tcgctcacga tcggaattgc atccataact
    11341 ttgagtctac tgaacctaga tacatcataa actattatca tatttagtcg accggcccga
    11401 cactcattta atattctttc aaattcaggc cgttcagcag taccaaaggc tgatgtacca
    11461 ggagcctcag agaaatgacc cacaaatcta aacctacccc catctctctc tacctctctt
    11521 tgtaaatcag cagctttatc ctcgttggca gatctctgtg tagctggaga ggcggcagaa
    11581 gagttctctc tttctcttga ttgtctatca taagcgccgg catacgtgtc catttcgact
    11641 agaatagtaa attgtaatgt tgtttgttgt ttgttttgtt gtggtaattg ttgtaaaaat
    11701 accggagtcc tctccaaatg aaatgaactt cctt
//
