LOCUS       sequence-32167-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Baculovirus transfer vector with His tag in 22-aa N-terminal 
            fusion peptide with TEV protease cleavage site. Includes sites for 
            LIC cloning, and SacB gene for negative selection .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pFB-LIC-Bse
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             118..138
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(168..201)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        216..661
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatctcggt
       61 ccgaaaacca tgggccacca tcatcatcat cattcttctg gtgtagatct gggtaccgag
      121 aacctgtact tccaatccat aagctagctt ctcctcctga aagatccata acttcgtata
      181 gcatacatta tacgaagtta tgcggccgcg acgtccacat atacctgccg ttcactatta
      241 tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc aactttatgc
      301 ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag attttttagt
      361 tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa agaggaaaat
      421 agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg
      481 gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt gtatactttg
      541 gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact tgccatcttc
      601 aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg agacatgaac
      661 gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta ccgcactgct
      721 ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat ataaggaaac
      781 atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac agcaaaaaa
//
