LOCUS       sequence-32168-e         462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Baculovirus transfer vector with His tag in 22-aa N-terminal 
            fusion peptide with TEV protease cleavage site. Includes sites for 
            LIC cloning, and SacB gene for negative selection .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pFB-LIC-Bse
FEATURES             Location/Qualifiers
     source          1..462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aacctgtact tccaatccat aagctagctt ctcctcctga aagatccata acttcgtata
       61 gcatacatta tacgaagtta tgcggccgcg acgtccacat atacctgccg ttcactatta
      121 tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc aactttatgc
      181 ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag attttttagt
      241 tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa agaggaaaat
      301 agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg
      361 gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt gtatactttg
      421 gcgtcacccc ttacatatnt taggtctttt tttattgtgc gt
//
