LOCUS       sequence-321692-        9699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of Ms2 fused to TV (TALx6 - 
            VP128) activation domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M,
            Garcia-Carpintero V, Granell A, Orzaez D
  TITLE     Strong gene activation in plants with genome-wide specificity using 
            a new orthogonal CRISPR/Cas9-based programmable transcriptional 
            activator.
  JOURNAL   Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 
            10.1111/pbi.13138. Epub 2019 May 23.
  PUBMED    31034138
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13138";
            journalName: "Plant Biotechnol J"; date: "2019-09"; volume: "17"; 
            issue: "9"; pages: "1703-1705"
COMMENT     Sequence Label: pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)
FEATURES             Location/Qualifiers
     source          1..9699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1061..1447
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1454..1474
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1478..1498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2537..2686
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2699..2848
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      2875..3127
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3162..3180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3364..3388
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4689..5318
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5747..6820
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6886..7080
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7316..7338)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7424..7564
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7479..7498
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7579..7596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7750..8338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7830..7849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8426..9241)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8426..9241)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9149..9168
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9674..9698
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca
     1021 acaaacaaca ttacaattta ctattctagt cgaaatgatg gcttctaatt ttactcaatt
     1081 tgttcttgtt gataatggtg gtactggtga tgttactgtt gctccttcta attttgctaa
     1141 tggtgttgct gaatggattt cttctaattc tagatctcaa gcttataagg ttacttgttc
     1201 tgttagacaa tcttctgctc aaaatagaaa gtatactatt aaggttgaag ttcctaaggt
     1261 tgctactcaa actgttggtg gtgttgaact tcctgttgct gcttggagat cttatcttaa
     1321 tatggaactt actattccta tttttgctac taattctgat tgtgaactta ttgttaaggc
     1381 tatgcaaggt cttcttaagg atggtaatcc tattccttct gctattgctg ctaattctgg
     1441 tatttattcg gacccgaaga aaaagaggaa ggtagatcct aagaagaaga gaaaggtact
     1501 gttggatccg ggaacaccta tggacgctga tttggtagct tcttctacag tagtgtggga
     1561 acaagatgca gatccttttg ctggaacagc cgacgacttc cccgccttca atgaagagga
     1621 gcttgcatgg ttgatggagc tgttacctca aggaggatca gggggtcttt tagatccagg
     1681 tactcccatg gatgcagacc tggtcgcttc aagtacagtc gtttgggagc aggatgctga
     1741 tccattcgca ggtacagcag atgacttccc ggctttcaat gaggaggaac ttgcttggct
     1801 catggaattg ctcccgcagg caagaggtgg atctggtggt ctgctcgacc caggaacgcc
     1861 gatggacgct gatctggtag catctagtac agtctgggag caggacgctg acccattcgc
     1921 tggtacggct gacgattttc ctgcctttaa tgaggaagag ttagcctggc tgatggagtt
     1981 attaccccag ggcggtagtg gcggattatt ggaccctggt acgccgatgg atgcagacct
     2041 tgtggcttca tcaacggtcg tatgggagca ggatgccgat cctttcgcag gaaccgcaga
     2101 cgacttccca gcctttaacg aagaagagct cgcatggctt atggaacttt tacctcaggc
     2161 acgtggagga agtggtgggc tcctggatcc aggcactccg atggacgcag acttagtagc
     2221 tagttccacc gttgtctggg agcaggatgc agatcctttt gctggtaccg cagacgactt
     2281 ccctgctttc aatgaagaag aattggcctg gcttatggaa ttacttccgc agggtgggtc
     2341 tggtgggctg ctcgatcccg gtacgccgat ggatgctgac ttggttgcta gcagtactgt
     2401 ggtctgggaa caggatgctg accccttcgc tgggactgcc gacgacttcc cagccttcaa
     2461 cgaagaggaa ctcgcatggt tgatggaact gcttcctcaa gcacgtgggg ggtcaggcgg
     2521 aggcggatct ggcggtgacg cactggacga ctttgatctc gacatgcttg ggtccgatgc
     2581 tctcgacgat tttgacctcg atatgcttgg gtctgacgca ttagatgact ttgatttaga
     2641 catgttgggg tcagacgctt tggacgattt cgacttagat atgttggcca gaggtagtga
     2701 tgcccttgac gacttcgatt tagatatgtt gggctctgac gccctcgatg acttcgatct
     2761 ggatatgctc ggaagtgacg ccttggacga tttcgacttg gacatgcttg ggtcggacgc
     2821 cctcgacgac tttgatctgg atatgttatg agcttggaat ggatcttcga tcccgatcgt
     2881 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgacgatt
     2941 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     3001 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     3061 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     3121 ctagatcggg aattgccaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
     3181 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3301 catgagacaa taaccctgat aaatgcttca ataatgggac cgactcgcgc ttgagacgaa
     3361 gcttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3421 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3481 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3541 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     3601 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3661 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3721 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3781 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3841 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3901 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3961 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     4021 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     4081 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     4141 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4201 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4261 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4321 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4381 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4441 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     4501 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4561 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4621 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     4681 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4741 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4801 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4861 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4921 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4981 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     5041 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     5101 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     5161 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5221 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5281 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5341 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5401 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5461 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5521 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5581 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5641 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     5701 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     5761 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     5821 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     5881 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     5941 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     6001 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     6061 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     6121 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     6181 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     6241 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     6301 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     6361 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     6421 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     6481 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     6541 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     6601 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     6661 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     6721 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     6781 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     6841 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta
     6901 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     6961 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     7021 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     7081 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     7141 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     7201 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     7261 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     7321 gacacatgca gctcccggtg acggtcacag cttgtctgta agcggatgcc gggagcagac
     7381 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     7441 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     7501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7561 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8401 gattttggtc atgcattcta ggtgattaga aaaactcatc gagcatcaaa tgaaactgca
     8461 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     8521 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     8581 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8641 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     8701 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8761 ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa
     8821 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8881 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     8941 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     9001 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     9061 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt
     9121 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     9181 catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca
     9241 taacagaact tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     9301 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     9361 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     9421 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     9481 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     9541 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     9601 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     9661 gttggctggc tggtggcagg atatattgtg gtgtaaaca
//
