LOCUS       sequence-321694-        7943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein interaction screening tool with Gateway-compatible BiFC 
            vectors comprising of a set of 4 vectors permiting N or C fusions of
            candidate genes to the N or C fragment of Split-Venus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Lepur A, Kovacevic L, Beluzic R, Vugrek O
  TITLE     Combining Unique Multiplex Gateway Cloning and Bimolecular 
            Fluorescence Complementation (BiFC) for High-Throughput Screening of
            Protein-Protein Interactions.
  JOURNAL   J Biomol Screen. 2016 Dec;21(10):1100-1111. doi: 
            10.1177/1087057116659438. Epub 2016 Jul 28.
  PUBMED    27455993
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1177/1087057116659438"; journalName: "J Biomol Screen"; date: 
            "2016-12"; volume: "21"; issue: "10"; pages: "1100-1111"
COMMENT     Sequence Label: pVenusCfN
FEATURES             Location/Qualifiers
     source          1..7943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(834..938)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1048..1351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1352..1555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1506..1526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1552..1576
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1673..1797
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1822..1827,1828..1845,1846..1852)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1906..2565
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARPMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1972..1991)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2907..3212
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3091..3110
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3253..3377)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      3397..3406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3403..3405,3406..3408,3409..3867)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(3448..3469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3709..3728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3905..3924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3906..3924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3935..3952)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3935..3951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3944..3966)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    4356..4490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4393..4412)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4447..4466
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4616..5071
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4703..4722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4913..4934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5188..5545
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5188..5379
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5211..5231)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5396..5531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5596..6390
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLVTTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5650..5669)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6260..6279
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6454..6502
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6543..6562)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6662..6684
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6808..6912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6913..6981,6982..7773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPDDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7131..7150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg gtaccgctcg
      721 ccgcagccgt acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg
      841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      961 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtattacg
     1021 aggccctttc acgcattaga tgcatgtcgt tacataactt acggtaaatg gcccgcctgg
     1081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1201 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1261 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1501 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     1561 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     1621 ccgggaccga tccagcctcc ggactctagc ctaggccgcg gagctcgagt caacaagttt
     1681 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     1741 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
     1801 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     1861 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1921 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1981 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2041 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2101 ccgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     2161 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2221 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2281 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     2341 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2401 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2461 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2521 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc tggatccggc
     2581 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     2641 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     2701 cagtgacagt tgacagcgat agctatcagt tgctcaaggc atatatgatg tcaatatctc
     2761 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     2821 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     2881 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     2941 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     3001 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     3061 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     3121 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     3181 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     3241 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     3301 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     3361 ttcttgtaca aagtggttga taccggtcgg agtatagcca ccatggtgag caagggcgag
     3421 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3481 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3541 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
     3601 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     3661 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3721 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3781 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3841 aacagccaca acgtctatat caccgccgac aagcagacgc gtgcatgaga cgtcatagct
     3901 ctctccctat agtgagtcgt attataagct aggcactggc cgtcgtttta caacgtcgtg
     3961 actgggaaaa ctgctagctt gggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     4021 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     4081 tataatgtgt taaactagct gcatatgctt gctgcttgag agttttgctt actgagtatg
     4141 atttatgaaa atattataca caggagctag tgattctaat tgtttgtgta ttttagattc
     4201 acagtcccaa ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca
     4261 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4321 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4381 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4441 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatcctgcat
     4501 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctggcg taatagcgaa
     4561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5041 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     5101 tgtgcggtac ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc
     5161 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     5221 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5281 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5341 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5401 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5461 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5521 ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt
     5581 aaggctagag ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5641 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5701 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5761 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggtcac gacgggcgtt
     5821 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggattggct gctattgggc
     5881 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5941 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6001 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6061 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6121 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6181 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6241 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6301 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6361 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6421 aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat
     6481 ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tccgcgtatg gtgcactctc
     6541 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     6601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     6721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaga cgacgagcgt
     7441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgga gatccttttt
     7861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7921 gtagaaaaga tcaaaggatc ttc
//
