LOCUS       sequence-321702-       11005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express 4X sGCN4 module in the G-baToN system
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Tang R, Murray CW, Linde IL, Kramer NJ, Lyu Z, Tsai MK, Chen LC, Cai
            H, Gitler AD, Engleman E, Lee W, Winslow MM
  TITLE     A versatile system to record cell-cell interactions.
  JOURNAL   Elife. 2020 Oct 7;9. pii: 61080. doi: 10.7554/eLife.61080.
  PUBMED    33025906
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-7"; volume: "9. pii: 61080. doi"; pages: " 
            10.7554/eLife.61080"
COMMENT     Sequence Label: pLVX-RT006-GCN4-HaloPM
FEATURES             Location/Qualifiers
     source          1..11005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(173..277)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    325..459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(362..381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     416..435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             627..1260
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1307..1432
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1929..2162
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2347..2391
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2654..2769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2963..4145
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3194..4136
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4093..4113
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4176..4185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4248..4304
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4320..4376
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4392..4448
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4464..4520
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4539..5429
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             5590..5643
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5650..5659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5656..6366
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5801..5819)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6069..6088
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(6381..6955,6956..6958,6959..6967)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6548..6565)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6775..6794
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6987..7586
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6987..7006)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7483..7503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7600..8188
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7653..7673)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8071..8082)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8395..9028
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9156..9172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9156..9172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9169..9191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9180..9196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9204..9210,9211..9228,9229..9234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9249..9270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9387..9404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9558..10146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9638..9657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10317..11005,1..103,104..172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10940..10959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      301 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
      361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      421 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
      481 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
      541 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
      601 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
      661 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
      721 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
      781 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
      841 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
      901 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
      961 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     1021 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     1081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1261 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1321 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1381 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1441 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1501 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1561 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1621 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1681 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1741 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     1801 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1861 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1921 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1981 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2041 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2101 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2161 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2221 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2281 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2341 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2401 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2461 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2521 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2581 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2641 cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2701 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2761 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat gagtaattca
     2821 tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa
     2881 cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg
     2941 aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3001 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     3061 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3121 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3181 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3241 tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc
     3301 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     3361 cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt
     3421 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     3481 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     3541 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3601 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3661 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3721 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3781 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3841 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3901 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3961 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     4021 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     4081 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     4141 cgtgaggatc tatttccggt gaattaggat ccgccgccac catggagacc gataccctcc
     4201 tgctgtgggt tctcctgctg tgggtgcctg gctccaccgg tgatatggaa gagctcctgt
     4261 caaaaaacta ccaccttgaa aacgaagtcg ccaggcttaa aaagggtagc ggatctgggg
     4321 aagagctgct gtccaaaaat taccaccttg agaatgaagt cgcgaggctc aaaaaaggta
     4381 gcggaagcgg agaggagctc ctttctaaaa attaccattt ggaaaatgag gtggcccggc
     4441 tcaaaaaggg ctccggtagc ggggaagaat tgttgagtaa gaactaccac ttggaaaacg
     4501 aggtagcacg acttaaaaaa ggcagcggat ctggctccat ggcagaaatc ggtactggct
     4561 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     4621 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     4681 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
     4741 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     4801 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     4861 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     4921 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
     4981 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
     5041 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     5101 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     5161 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     5221 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     5281 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     5341 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     5401 gctggctgtc gacgctcgag atttccggcg ggtccgggag cggcccatag tcaggaggtg
     5461 accgtcgtgc ctcactccct gccctttaag gtggtcgtta tctcagctat acttgccctt
     5521 gtggttttga ctgttatatc cctgattatc ctcatcatgc tgtggcagaa aaagccccgg
     5581 ggcggccgcg agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     5641 ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     5701 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     5761 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     5821 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     5881 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     5941 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     6001 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     6061 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     6121 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6181 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     6241 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     6301 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6361 aagtaatcta gaagatcccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     6421 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     6481 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     6541 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     6601 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     6661 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     6721 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     6781 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     6841 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     6901 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     6961 cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca
     7021 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     7081 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7141 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7201 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7261 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7321 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     7381 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7441 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7501 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7561 gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa
     7621 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7681 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7741 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7801 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7861 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7921 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7981 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     8041 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8101 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8161 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     8221 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     8281 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     8341 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     8401 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     8461 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     8521 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     8581 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     8641 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     8701 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     8761 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     8821 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8881 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8941 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9001 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     9061 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
     9121 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10981 attggaaaac gttcttcggg gcgaa
//
