LOCUS       sequence-321706-       10536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express sGFP module in the G-baToN system
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Tang R, Murray CW, Linde IL, Kramer NJ, Lyu Z, Tsai MK, Chen LC, Cai
            H, Gitler AD, Engleman E, Lee W, Winslow MM
  TITLE     A versatile system to record cell-cell interactions.
  JOURNAL   Elife. 2020 Oct 7;9. pii: 61080. doi: 10.7554/eLife.61080.
  PUBMED    33025906
REFERENCE   2  (bases 1 to 10536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-7"; volume: "9. pii: 61080. doi"; pages: " 
            10.7554/eLife.61080"
COMMENT     Sequence Label: pLVX-RT003-GPM
FEATURES             Location/Qualifiers
     source          1..10536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(173..277)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    325..459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(362..381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     416..435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             627..1260
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1307..1432
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1929..2162
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2347..2391
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2654..2769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2963..4145
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3194..4136
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4093..4113
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4176..4185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4245..4247,4248..4250,4251..4961)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5121..5174
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5181..5190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5187..5897
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5332..5350)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5600..5619
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(5912..6486,6487..6489,6490..6498)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6079..6096)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6306..6325
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6518..7117
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6518..6537)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7014..7034
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7131..7719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7184..7204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7602..7613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7926..8559
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8687..8703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8687..8703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8700..8722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8711..8727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8735..8741,8742..8759,8760..8765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8780..8801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8918..8935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9089..9677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9169..9188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9848..10536,1..103,104..172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10471..10490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      301 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
      361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      421 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
      481 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
      541 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
      601 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
      661 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
      721 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
      781 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
      841 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
      901 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
      961 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     1021 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     1081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1261 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1321 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1381 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1441 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1501 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1561 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1621 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1681 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1741 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     1801 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1861 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1921 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1981 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2041 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2101 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2161 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2221 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2281 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2341 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2401 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2461 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2521 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2581 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2641 cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2701 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2761 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat gagtaattca
     2821 tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa
     2881 cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg
     2941 aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3001 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     3061 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3121 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3181 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3241 tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc
     3301 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     3361 cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt
     3421 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     3481 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     3541 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3601 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3661 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3721 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3781 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3841 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3901 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3961 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     4021 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     4081 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     4141 cgtgaggatc tatttccggt gaattaggat ccgccgccac catggagacc gataccctcc
     4201 tgctgtgggt tctcctgctg tgggtgcctg gctccaccgg tgatatggtt agcaagggcg
     4261 aagagctctt tactggcgtc gtgccaatac tcgtcgagct ggatggggac gttaatggcc
     4321 ataaattcag cgtgagcggc gagggggagg gggacgccac ctacggaaag cttactttga
     4381 agtttatttg cactacaggc aagttgcctg tgccttggcc tacactcgtg accacactca
     4441 cttacggggt gcagtgtttt tctaggtatc ctgatcacat gaaacagcac gactttttca
     4501 agagcgcaat gcctgaaggc tacgtccagg agagaaccat atttttcaag gatgatggta
     4561 actacaaaac tagagctgaa gtcaagtttg agggggacac cctcgtgaac agaattgaat
     4621 tgaaaggcat tgatttcaag gaggacggaa acattctcgg acacaaactg gaatataatt
     4681 acaatagtca taacgtctat atcatggcag ataagcagaa gaacgggatt aaagtcaatt
     4741 tcaaaatcag acacaatatc gaggatggct ccgttcaact ggctgatcat tatcaacaga
     4801 acacccctat cggcgacgga cctgttttgc tccctgacaa tcactacttg tctacccagt
     4861 ccgctctcag caaagacccc aacgaaaagc gcgatcacat ggttctgctg gagttcgtga
     4921 cagccgcagg cataacactg gggatggacg agctttacaa gaattcgggt ttaaacgata
     4981 gtcaggaggt gaccgtcgtg cctcactccc tgccctttaa ggtggtcgtt atctcagcta
     5041 tacttgccct tgtggttttg actgttatat ccctgattat cctcatcatg ctgtggcaga
     5101 aaaagccccg gggcggccgc gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     5161 agaatcctgg cccaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
     5221 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     5281 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     5341 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     5401 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     5461 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     5521 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     5581 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     5641 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     5701 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     5761 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     5821 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     5881 acgagctgta caagtaatct agaagatccc gcccctctcc ctcccccccc cctaacgtta
     5941 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     6001 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     6061 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     6121 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     6181 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     6241 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     6301 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     6361 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     6421 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6481 gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt
     6541 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     6601 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     6661 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6721 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6781 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6841 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6901 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6961 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7021 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7081 gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac aatcaacctc
     7141 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7201 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7261 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7321 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7381 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7441 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7501 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     7561 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7621 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7681 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7741 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7801 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7861 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     7921 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     7981 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     8041 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8101 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8161 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8221 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8281 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8341 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8401 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8461 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8521 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8581 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     8641 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10501 aagtgctcat cattggaaaa cgttcttcgg ggcgaa
//
