LOCUS       sequence-321707-       11445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral vector to express sHalotag-sGFP module in 
            the G-baToN system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Tang R, Murray CW, Linde IL, Kramer NJ, Lyu Z, Tsai MK, Chen LC, Cai
            H, Gitler AD, Engleman E, Lee W, Winslow MM
  TITLE     A versatile system to record cell-cell interactions.
  JOURNAL   Elife. 2020 Oct 7;9. pii: 61080. doi: 10.7554/eLife.61080.
  PUBMED    33025906
REFERENCE   2  (bases 1 to 11445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-7"; volume: "9. pii: 61080. doi"; pages: " 
            10.7554/eLife.61080"
COMMENT     Sequence Label: pLVX-RT005-HaloGPM
FEATURES             Location/Qualifiers
     source          1..11445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(173..277)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    325..459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(362..381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     416..435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             627..1260
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1307..1432
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1929..2162
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2347..2391
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2654..2769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2963..4145
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3194..4136
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4093..4113
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4176..4185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4245..5135
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             join(5154..5156,5157..5159,5160..5870)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6030..6083
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6090..6099
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6096..6806
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6241..6259)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6509..6528
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(6821..7395,7396..7398,7399..7407)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6988..7005)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7215..7234
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7427..8026
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7427..7446)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7923..7943
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8040..8628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8093..8113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8511..8522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8835..9468
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9596..9612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9596..9612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9609..9631)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9620..9636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9644..9650,9651..9668,9669..9674))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9689..9710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9827..9844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9998..10586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10078..10097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10757..11445,1..103,104..172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11380..11399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      301 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
      361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      421 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
      481 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
      541 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
      601 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
      661 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
      721 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
      781 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
      841 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
      901 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
      961 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     1021 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     1081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1261 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1321 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1381 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1441 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1501 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1561 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1621 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1681 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1741 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     1801 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1861 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1921 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1981 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2041 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2101 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2161 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2221 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2281 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2341 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2401 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2461 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2521 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2581 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2641 cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2701 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2761 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat gagtaattca
     2821 tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa
     2881 cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg
     2941 aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3001 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     3061 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3121 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3181 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3241 tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc
     3301 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     3361 cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt
     3421 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     3481 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     3541 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3601 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3661 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3721 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3781 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3841 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3901 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3961 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     4021 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     4081 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     4141 cgtgaggatc tatttccggt gaattaggat ccgccgccac catggagacc gataccctcc
     4201 tgctgtgggt tctcctgctg tgggtgcctg gctccaccgg tgatatggca gaaatcggta
     4261 ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg
     4321 atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct
     4381 acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc
     4441 tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc
     4501 gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg
     4561 actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta
     4621 ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc
     4681 gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga
     4741 acgtttttat cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga
     4801 tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc
     4861 caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca
     4921 tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc ccaggcgttc
     4981 tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg
     5041 acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga
     5101 tcgcgcgctg gctgtcgacg ctcgagattt ccggcggtag tggtagcggc agcatggtta
     5161 gcaagggcga agagctcttt actggcgtcg tgccaatact cgtcgagctg gatggggacg
     5221 ttaatggcca taaattcagc gtgagcggcg agggggaggg ggacgccacc tacggaaagc
     5281 ttactttgaa gtttatttgc actacaggca agttgcctgt gccttggcct acactcgtga
     5341 ccacactcac ttacggggtg cagtgttttt ctaggtatcc tgatcacatg aaacagcacg
     5401 actttttcaa gagcgcaatg cctgaaggct acgtccagga gagaaccata tttttcaagg
     5461 atgatggtaa ctacaaaact agagctgaag tcaagtttga gggggacacc ctcgtgaaca
     5521 gaattgaatt gaaaggcatt gatttcaagg aggacggaaa cattctcgga cacaaactgg
     5581 aatataatta caatagtcat aacgtctata tcatggcaga taagcagaag aacgggatta
     5641 aagtcaattt caaaatcaga cacaatatcg aggatggctc cgttcaactg gctgatcatt
     5701 atcaacagaa cacccctatc ggcgacggac ctgttttgct ccctgacaat cactacttgt
     5761 ctacccagtc cgctctcagc aaagacccca acgaaaagcg cgatcacatg gttctgctgg
     5821 agttcgtgac agccgcaggc ataacactgg ggatggacga gctttacaag aattcgggtt
     5881 taaacgatag tcaggaggtg accgtcgtgc ctcactccct gccctttaag gtggtcgtta
     5941 tctcagctat acttgccctt gtggttttga ctgttatatc cctgattatc ctcatcatgc
     6001 tgtggcagaa aaagccccgg ggcggccgcg agggcagagg aagtctgcta acatgcggtg
     6061 acgtcgagga gaatcctggc ccaccggtcg ccaccatggt gagcaagggc gaggaggata
     6121 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     6181 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     6241 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     6301 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     6361 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     6421 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     6481 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     6541 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     6601 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     6661 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     6721 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     6781 gcggcatgga cgagctgtac aagtaatcta gaagatcccg cccctctccc tccccccccc
     6841 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     6901 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     6961 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     7021 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     7081 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     7141 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     7201 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     7261 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     7321 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     7381 aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa
     7441 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     7501 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     7561 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7621 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7681 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7741 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7801 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7861 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7921 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7981 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc gtctggaaca
     8041 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8101 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8161 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8221 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8281 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8341 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8401 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     8461 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8521 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8581 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     8641 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     8701 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     8761 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8821 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     8881 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     8941 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     9001 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     9061 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     9121 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     9181 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     9241 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     9301 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9361 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9421 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     9481 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     9541 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     9601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9661 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaa
//
