LOCUS       sequence-32171-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, 
            Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB,
            van de Vijver MJ, Sukumar S, Chang HY
  TITLE     Long non-coding RNA HOTAIR reprograms chromatin state to promote 
            cancer metastasis.
  JOURNAL   Nature. 2010 Apr 15. 464(7291):1071-6.
  PUBMED    20393566
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Apr 15. 464(7291):1071-6."
COMMENT     Sequence Label: LZRS-Flag_EZH2_ER
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(408..431)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      431..440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(443..467)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(543..559)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 tctcccatat aaggaatgtt atgtaaaaca gtttcatctt ccaccataaa attctgctgt
       61 aggggagacc aagaatacat tatgggtact gaagcaactg cattcagagt ctttaatggg
      121 atgacttgtg ttggaaaatc caagtcactg gtcaccgaac actccctagt cccgcgcaat
      181 gagctcacag aagtcaggat gtgcacaggc tgtatccttc gctgtttcca ttcttggttt
      241 aagatttccg ttctttccaa aattttctga cgattggaac taaacatact ctttacttca
      301 tcagctcgtc tgaacctctt gagctgtctc agtcgcatgt actctgattt tacacgcttc
      361 cgccaacaaa ctggtccctt ctcagatttc ttcccagtct ggcccatctt gtcatcgtcg
      421 tccttgtaat ccatggtggc gaagcctgct tttttgtaca aacttgtgat tcccgtacca
      481 ccacactggg atccngcagt ctagaggatg gtccaccccc ggggtcggca gccttcacgt
      541 gggcggcgtg tatccaagct gcgatgccgt ctactttgag ggcggtgggg gtggtcagca
      601 ggactgtgta aggtcctttc cagcgaggtt ctaggttctt agtctggtgt cggcggaccc
      661 acactgtgtc gccgactcgg taagggtgag gtaccaccgg tcggtccagt tgttcttggt
      721 aggctgccgc cagaggtctc cagacttcgt gctggactaa gtagagagcc tgtaagtgag
      781 cttggagaga gggggctg
//
