LOCUS       sequence-32173-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     pNIC-CH
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pNIC-CH
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(95..112)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcgggctttg ttagcagccg ganctcagtg gtggtggtgg tggtgctcga gtgcggccgc
       61 aagcttgtcg acggagctcg aattcggatc ctcaatggtg gtgatgatgg tgcgctgcag
      121 gtgtgccacc tgacgtcaat gccaatagga tatcggcatt ttcttttgcg tttttatttg
      181 ttaactgtta attgtccttg ttcaaggatg ctgtctttga caacagatgt tttcttgcct
      241 ttgatgttca gcaggaagct aggcgcaaac gttgattgtt tgtctgcgta gaatcctctg
      301 tttgtcatat agcttgtaat cacgacattg tttcctttcg cttgaggtac agcgaagtgt
      361 gagtaagtaa aggttacatc gttaggatca agatccattt ttaacacaag gccagttttg
      421 ttcagcggct tgtatgggcc agttaaagaa ttagaaacat aaccaagcat gtaaatatcg
      481 ttagacgtaa tgccgtcaat cgtcattttt gatccgcggg agtcagtgaa caggtaccat
      541 ttgccgttca ttttaaagac gttcgcgcgt tcaatttcat ctgttactgt gttagatgca
      601 atcagcggtt tcatcacttt tttcagtgtg taatcatcgt ttagctcaat cataccgaga
      661 gcgccgtttg ctaactcagc cgtgcgtttt ttatcgcttt gcagaagttt ttgactttct
      721 tgacggaaga atgatgtgct tttgccatag tatgctttgt taaataaaga ttcttcgcct
      781 tggtagccat cttcagttcc agtgtttgct tcaaatacta agtatttgtg gcctttatct
      841 tctacgtagt gaggatctct cagcgtatgg ttgtcgcctg agctgtagtt gccttcatcg
      901 atgaactgct gtacatt
//
