LOCUS       sequence-32177-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF4
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(260..278)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(261..278)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(298..315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    304..528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(661..680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     871..892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctggataaga agctgaaaaa aatggagcaa aacaagacag cagccactag gtaccgccag
       61 aagaagaggg cggagcagga ggctcttact ggtgagtgca aagagctgga aaagaagaac
      121 gaggctctaa aagagagggc ggattccctg gccaaggaga tccagtacct gaaagatttg
      181 atagaagagg tccgcaaggc aagggggaag aaaagggtcc cctaggcggc cgctcgagca
      241 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
      301 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      361 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      421 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      481 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      541 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
      601 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      661 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      721 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      781 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      841 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      901 tcaaccctat ctcggtcta
//
