LOCUS       sequence-32180-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF3
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(359..382)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      382..391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(431..450)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(463..481)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(464..481)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(691..715)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(712..811)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(741..761)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctttctgcag gcactccgtc ttctccttct tcttgtttcg gcactttgca gctgcaatct
       61 tatttctttc tcgtcgcctc tttttccttt catcttcttc aggggctacc tcggcttttg
      121 tgatggacac cccgaggggt ctgtcgctga cagtgactga ttccagcgca gaggacatcc
      181 ggtggcagag gtgcttgttc tggatggcaa acctcagctc ttccttgaca aagggcgtca
      241 ggttagcaaa atcctcaaac accagtgacc caggagggga caggcagggg acgatggcag
      301 aagcactcac ttccgaggca gagacctggc ctgggtgttg aagcatcatg gtcgacccct
      361 tgtcatcgtc gtccttgtag tccatggcgg caagcttaga accgaggtgc agttggacct
      421 gggagtggac acctgtggag agaaaggcaa agtggatgtt attctatagt gtcacctaaa
      481 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgaagccaa
      541 gggggtgggc ctatagactc tataggcggt acttacgtca ctcttggcac ggggaatccg
      601 cgttccaatg caccgttccc ggccgcggag gctggatcgg tcccggtgtc ttctatggag
      661 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct
      721 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      781 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      841 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      901 cc
//
