LOCUS       sequence-321827-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ostir and G418 resistance gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J
  TITLE     Opposing Effects of Cohesin and Transcription on CTCF Organization 
            Revealed by Super-resolution Imaging.
  JOURNAL   Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi: 
            10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21.
  PUBMED    33091336
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10.001"; journalName: "Mol Cell"; date: 
            "2020-11-19- 19"; volume: "80"; issue: "4"; pages: "699-711.e7"
COMMENT     Sequence Label: PB-Ostir-neo
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1755..3479
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3480..3560
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             3570..3626
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3633..4424
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3684..3703)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4294..4313
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4452..4469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4458..4665
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4681..4802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4718..4737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4772..4791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5565..5599
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6090..6106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6090..6106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6103..6125)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6114..6130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6138..6144,6145..6162,6163..6168))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6183..6204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6321..6338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6492..7080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6572..6591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7251..8042,8043..8111))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7874..7893
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8112..8216)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8242..8697)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8379..8400)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8591..8610
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8824..8846
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8838..8855
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8839..8855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8985..9047
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      421 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      481 gatgggggcg gggggggggg gggggsgsgc srggsggggs ggggsggggs grggggsggg
      541 gsggggsgrg gsggrgrggk gsggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggk ggcggcrggk gggggkgccg ggcggggcgg
     1261 ggccgcckcg ggcsggggrg ggckcggggg rggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgagaatt
     1741 ctcacgcgtc caccatgaca tacttccctg aggaggtggt cgaacatatt ttctcattcc
     1801 ttcccgccca acgagatagg aataccgtgt ccctggtgtg caaggtctgg tatgagatcg
     1861 aacgcttgag ccgcagaggt gtttttgtgg gtaattgtta tgccgtacga gccgggcgag
     1921 tggccgcccg gttcccgaac gtcagggccc ttacagtgaa gggcaagcct cactttgccg
     1981 acttcaattt ggtgcctccg gactggggcg gctacgccgg gccctggatc gaggccgctg
     2041 cgcgcgggtg tcatggtctg gaagaactca ggatgaaaag gatggtggtg tcagacgagt
     2101 cactggaact gcttgcccgc agcttccccc gattccgcgc cctggtgctg atcagctgtg
     2161 aaggatttag caccgacggt ctggctgctg tagctagcca ttgtaaactg ctgcgagaac
     2221 tggacctgca ggaaaatgag gtagaggata ggggacctcg gtggctgagc tgtttcccgg
     2281 atagctgcac tagcctggtg agcttgaatt tcgcttgtat caagggcgaa gtgaatgccg
     2341 ggagtttgga gagactcgtt tcccgatccc ccaacctgag atctctgcgg ctgaacagat
     2401 ccgtttctgt ggacacgctc gccaagattt tgctccggac tcccaacctg gaggatctgg
     2461 ggaccggaaa tctgaccgat gatttccaga cagagagcta ctttaagctc actagtgctc
     2521 tggaaaaatg caaaatgctt aggtcactga gcgggttttg ggacgctagc cctgtctgcc
     2581 tgtcttttat ctatcccctg tgtgcacagt tgaccggact taatctgagt tacgctccaa
     2641 ccctcgacgc tagtgacctt actaagatga tcagccgctg cgtgaagctc caaaggctct
     2701 gggtactgga ttgtatttca gacaagggac tgcaagttgt ggcctcctcc tgcaaagatc
     2761 tgcaggagct gagggtgttc ccttcagatt tctatgtggc cggatacagc gcagtcaccg
     2821 aggagggatt ggtagcagtg tccctggggt gccccaaact caatagtttg ctgtacttct
     2881 gtcatcagat gaccaatgct gcccttgtta ccgttgccaa aaactgtcca aacttcacaa
     2941 ggtttagact gtgcatcttg gagcccggta agcccgacgt cgtaacgtca cagccactgg
     3001 acgaagggtt cggtgccata gtacgcgaat gtaagggtct ccagcggctg agcatatccg
     3061 gcctgctgac tgacaaagtg ttcatgtata ttgggaagta cgccaagcag ctggaaatgc
     3121 tgtcaatcgc attcgccgga gattcagata aagggatgat gcacgtgatg aacgggtgca
     3181 agaacctgag aaaactcgaa attcgcgatt ccccttttgg ggacgccgca ctgctgggca
     3241 atttcgcccg gtacgagacg atgaggtcac tttggatgtc aagctgcaac gtgaccctca
     3301 agggatgcca agtcctggct agtaaaatgc caatgctgaa tgttgaggtc atcaacgaac
     3361 gcgacggtag taatgaaatg gaggaaaacc acggcgatct gcccaaagtg gagaaactgt
     3421 acgtctatcg gaccacagct ggcgcacgag atgacgctcc caatttcgtg aaaattctgt
     3481 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat
     3541 atgatgtccc cgactatgcc ggaagcggag ctactaactt cagcctgctg aagcaggctg
     3601 gagacgtgga ggagaaccct ggacctaagc ttattgaaca agatggattg cacgcaggtt
     3661 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3721 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3781 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3841 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3901 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3961 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4021 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4081 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4141 tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg
     4201 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4261 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4321 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4381 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagaattc taactagagc
     4441 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4501 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4561 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4621 cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc caactaacta
     4681 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4741 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4801 tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct catctggtct
     4861 cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc ccaaactggg
     4921 ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga
     4981 agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga ttcctttttt
     5041 agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     5101 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     5161 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     5221 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     5281 ggctgtccct gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     5341 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     5401 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     5461 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     5521 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     5581 gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt
     5641 ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
     5701 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat
     5761 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
     5821 ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg
     5881 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag
     5941 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     6001 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt
     6061 acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6121 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6181 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6241 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6301 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6361 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6421 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6481 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6541 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6601 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6661 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6841 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6901 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6961 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7021 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7081 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7141 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7201 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7261 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7321 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7381 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7441 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7501 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7561 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7621 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7681 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7741 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7801 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7861 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7921 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7981 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8041 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8101 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8161 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8221 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     8281 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     8341 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     8401 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     8461 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     8521 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     8581 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     8641 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     8701 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8761 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8821 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     8881 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     8941 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     9001 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     9061 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     9121 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     9181 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     9241 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     9301 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     9361 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     9421 gagcctgcag acacctgggg ggatacgggg aaaaggcct
//
