LOCUS       sequence-321828-        9242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ostir and puromycin resistance gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J
  TITLE     Opposing Effects of Cohesin and Transcription on CTCF Organization 
            Revealed by Super-resolution Imaging.
  JOURNAL   Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi: 
            10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21.
  PUBMED    33091336
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10.001"; journalName: "Mol Cell"; date: 
            "2020-11-19- 19"; volume: "80"; issue: "4"; pages: "699-711.e7"
COMMENT     Sequence Label: PB-Ostir-puro
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          144..1160
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1084..1106
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1168..1187
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1239..2963
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             2964..3044
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             3054..3107
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3108..3707
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3108..3127)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(3735..3752)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3741..3948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3964..4085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4001..4020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4055..4074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4848..4882
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5373..5389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5373..5389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5386..5408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5397..5413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5421..5427,5428..5445,5446..5451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5466..5487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5604..5621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5775..6363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5855..5874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6534..7325,7326..7394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7157..7176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7395..7499)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7525..7980)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7662..7683)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7874..7893
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8107..8129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8121..8138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8122..8138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8268..8330
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        8745..9124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
       61 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      121 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      181 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      241 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      301 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
      361 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
      421 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
      481 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
      541 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
      601 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
      661 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
      721 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
      781 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
      841 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
      901 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
      961 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     1021 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     1081 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     1141 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1201 ttggcaaaga attcctcgag aattctcacg cgtccaccat gacatacttc cctgaggagg
     1261 tggtcgaaca tattttctca ttccttcccg cccaacgaga taggaatacc gtgtccctgg
     1321 tgtgcaaggt ctggtatgag atcgaacgct tgagccgcag aggtgttttt gtgggtaatt
     1381 gttatgccgt acgagccggg cgagtggccg cccggttccc gaacgtcagg gcccttacag
     1441 tgaagggcaa gcctcacttt gccgacttca atttggtgcc tccggactgg ggcggctacg
     1501 ccgggccctg gatcgaggcc gctgcgcgcg ggtgtcatgg tctggaagaa ctcaggatga
     1561 aaaggatggt ggtgtcagac gagtcactgg aactgcttgc ccgcagcttc ccccgattcc
     1621 gcgccctggt gctgatcagc tgtgaaggat ttagcaccga cggtctggct gctgtagcta
     1681 gccattgtaa actgctgcga gaactggacc tgcaggaaaa tgaggtagag gataggggac
     1741 ctcggtggct gagctgtttc ccggatagct gcactagcct ggtgagcttg aatttcgctt
     1801 gtatcaaggg cgaagtgaat gccgggagtt tggagagact cgtttcccga tcccccaacc
     1861 tgagatctct gcggctgaac agatccgttt ctgtggacac gctcgccaag attttgctcc
     1921 ggactcccaa cctggaggat ctggggaccg gaaatctgac cgatgatttc cagacagaga
     1981 gctactttaa gctcactagt gctctggaaa aatgcaaaat gcttaggtca ctgagcgggt
     2041 tttgggacgc tagccctgtc tgcctgtctt ttatctatcc cctgtgtgca cagttgaccg
     2101 gacttaatct gagttacgct ccaaccctcg acgctagtga ccttactaag atgatcagcc
     2161 gctgcgtgaa gctccaaagg ctctgggtac tggattgtat ttcagacaag ggactgcaag
     2221 ttgtggcctc ctcctgcaaa gatctgcagg agctgagggt gttcccttca gatttctatg
     2281 tggccggata cagcgcagtc accgaggagg gattggtagc agtgtccctg gggtgcccca
     2341 aactcaatag tttgctgtac ttctgtcatc agatgaccaa tgctgccctt gttaccgttg
     2401 ccaaaaactg tccaaacttc acaaggttta gactgtgcat cttggagccc ggtaagcccg
     2461 acgtcgtaac gtcacagcca ctggacgaag ggttcggtgc catagtacgc gaatgtaagg
     2521 gtctccagcg gctgagcata tccggcctgc tgactgacaa agtgttcatg tatattggga
     2581 agtacgccaa gcagctggaa atgctgtcaa tcgcattcgc cggagattca gataaaggga
     2641 tgatgcacgt gatgaacggg tgcaagaacc tgagaaaact cgaaattcgc gattcccctt
     2701 ttggggacgc cgcactgctg ggcaatttcg cccggtacga gacgatgagg tcactttgga
     2761 tgtcaagctg caacgtgacc ctcaagggat gccaagtcct ggctagtaaa atgccaatgc
     2821 tgaatgttga ggtcatcaac gaacgcgacg gtagtaatga aatggaggaa aaccacggcg
     2881 atctgcccaa agtggagaaa ctgtacgtct atcggaccac agctggcgca cgagatgacg
     2941 ctcccaattt cgtgaaaatt ctgtacccat acgatgttcc agattacgct tatccctacg
     3001 acgtgcctga ttatgcatac ccatatgatg tccccgacta tgccggcagt ggagagggca
     3061 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg accgagtaca
     3121 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     3181 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     3241 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     3301 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     3361 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3421 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     3481 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3541 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3601 cccacaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3661 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagaa ttctaactag
     3721 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3901 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccaactaa
     3961 ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4081 tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg
     4141 tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact
     4201 gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa
     4261 cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt
     4321 tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     4381 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     4441 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
     4501 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg
     4561 gggggctgtc cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt
     4621 agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga
     4681 taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg
     4741 cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag
     4801 cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt
     4861 tacgcagact atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt
     4921 tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg
     4981 tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg
     5041 cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca
     5101 cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt
     5161 ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga
     5221 cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta
     5281 cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga
     5341 gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5401 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5461 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5521 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5581 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5641 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5701 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5761 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5821 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5881 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5941 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6001 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6061 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6121 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6181 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6241 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6301 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6361 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6841 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6961 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7141 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7501 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     7561 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7621 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7681 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7741 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7801 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     7861 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     7921 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     7981 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8041 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8101 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg
     8161 tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg
     8221 agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata
     8281 atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg
     8341 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
     8401 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
     8461 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc
     8521 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     8581 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     8641 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     8701 tttgagcctg cagacacctg gggggatacg gggaaaaggc cttcgacatt gattattgac
     8761 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     8821 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     8881 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     8941 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     9001 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     9061 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     9121 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     9181 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     9241 gg
//
